Communications Biology (Jan 2022)

Demonstration of targeted crossovers in hybrid maize using CRISPR technology

  • Andrei Kouranov,
  • Charles Armstrong,
  • Ashok Shrawat,
  • Vladimir Sidorov,
  • Scott Huesgen,
  • Bryce Lemke,
  • Timothy Boyle,
  • Michelle Gasper,
  • Richard Lawrence,
  • Samuel Yang

DOI
https://doi.org/10.1038/s42003-022-03004-9
Journal volume & issue
Vol. 5, no. 1
pp. 1 – 11

Abstract

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Kouranov et al. demonstrate Cas12a-mediated targeted chromosomal crossover (CO) in transgenic hybrid maize plants and produce heritable crossover in F1 plants when Cas12a transgene is segregated away. The authors also report that the frequency of LbCas12a-mediated CO is higher than control natural CO in the same intervals in the progeny of 4 different events.