Single cell census of human kidney organoids shows reproducibility and diminished off-target cells after transplantation

Ayshwarya Subramanian; Eriene-Heidi Sidhom; Maheswarareddy Emani; Katherine Vernon; Nareh Sahakian; Yiming Zhou; Maria Kost-Alimova; Michal Slyper; Julia Waldman; Danielle Dionne; Lan T. Nguyen; Astrid Weins; Jamie L. Marshall; Orit Rosenblatt-Rosen; Aviv Regev; Anna Greka

doi:10.1038/s41467-019-13382-0

Nature Communications (Nov 2019)

Single cell census of human kidney organoids shows reproducibility and diminished off-target cells after transplantation

Ayshwarya Subramanian,
Eriene-Heidi Sidhom,
Maheswarareddy Emani,
Katherine Vernon,
Nareh Sahakian,
Yiming Zhou,
Maria Kost-Alimova,
Michal Slyper,
Julia Waldman,
Danielle Dionne,
Lan T. Nguyen,
Astrid Weins,
Jamie L. Marshall,
Orit Rosenblatt-Rosen,
Aviv Regev,
Anna Greka

Affiliations

Ayshwarya Subramanian: Broad Institute of MIT and Harvard
Eriene-Heidi Sidhom: Broad Institute of MIT and Harvard
Maheswarareddy Emani: Broad Institute of MIT and Harvard
Katherine Vernon: Broad Institute of MIT and Harvard
Nareh Sahakian: Broad Institute of MIT and Harvard
Yiming Zhou: Broad Institute of MIT and Harvard
Maria Kost-Alimova: Center for the Development of Therapeutics, Broad Institute of MIT and Harvard
Michal Slyper: Broad Institute of MIT and Harvard
Julia Waldman: Broad Institute of MIT and Harvard
Danielle Dionne: Broad Institute of MIT and Harvard
Lan T. Nguyen: Broad Institute of MIT and Harvard
Astrid Weins: Department of Pathology, Brigham and Women’s Hospital and Harvard Medical School
Jamie L. Marshall: Broad Institute of MIT and Harvard
Orit Rosenblatt-Rosen: Broad Institute of MIT and Harvard
Aviv Regev: Broad Institute of MIT and Harvard
Anna Greka: Broad Institute of MIT and Harvard

DOI: https://doi.org/10.1038/s41467-019-13382-0
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 15

Abstract

Read online

How reproducible human kidney organoids derived from different iPSC lines are, and how faithful they are to human kidney tissue remain unclear. Here, the authors use four human iPSC lines to derive kidney organoids and show how organoid composition is reproducible, comparable to human tissue and of improved quality after transplantation.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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