A primary attempt of Leptinotarsa decemlineata control using contact DNA insecticide based on short antisense oligonucleotide of its CYP6B gene

Abstract

Effective control of Leptinotarsa decemlineata remains an urgent problem for agriculture worldwide. Minimization of the use of non-selective neonicotinoid insecticides, such as thiomethoxam, is an actual vector of development of potato cultivation. In this rapid communication, we show the prospect of the topical use of short unmodified antisense fragment of L. decemlineata CYP6B gene as a DNA insecticide. Investigated parameters, namely, number of larvae per plant, aboveground biomass, yield and number of potatoes produced per plant indicate the possibility of this post-genomic approach as a safe and effective method of L. decemlineata control.

Keywords

• antisense oligonucleotide
• cytochrome P450 monooxygenase gene
• DNA insecticide
• Leptinotarsa decemlineata
• neonicotinoid insecticides
• potato