Identification of a novel hypotensive peptide from porcine plasma hydrolysate by in vitro digestion and rat model

Junqi Zhan; Gaoshang Li; Yali Dang; Daodong Pan

Food Chemistry: Molecular Sciences (Jul 2022)

Identification of a novel hypotensive peptide from porcine plasma hydrolysate by in vitro digestion and rat model

Junqi Zhan,
Gaoshang Li,
Yali Dang,
Daodong Pan

Affiliations

Junqi Zhan: State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo 315211, China; Key Laboratory of Animal Protein Food Processing Technology of Zhejiang Province, Ningbo University, Ningbo 315211, Zhejiang, China
Gaoshang Li: Institute of Food Engineering, Zhejiang University, Hangzhou 310058, Zhejiang, China
Yali Dang: State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo 315211, China; Key Laboratory of Animal Protein Food Processing Technology of Zhejiang Province, Ningbo University, Ningbo 315211, Zhejiang, China; Corresponding authors at: State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo 315211, China.
Daodong Pan: State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo 315211, China; Key Laboratory of Animal Protein Food Processing Technology of Zhejiang Province, Ningbo University, Ningbo 315211, Zhejiang, China; Corresponding authors at: State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo 315211, China.

Journal volume & issue: Vol. 4
p. 100101

Abstract

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We separated a novel functional peptide IFPPKPKDTL from porcine plasma hydrolysate by chromatography, HPLC, and identified by Q Exactive LC-MS/MS. Results showed that IFPPKPKDTL had a significant ability of ACE inhibition (76.6%) likely due to the presence of hydrophobic, aromatic, and acidic amino acids that can inactivate ACE by binding Zn2+, providing a hydrogen atom to maintain the link between ACE and the peptide. Furthermore, the ACE inhibition of synthetic IFPPKPKDTL was improved by 15.6% after in vitro digestion. Additionally, the systolic blood pressure and diastolic blood pressure of spontaneously hypertensive rats gavaged by the peptide (30 mg/kg). Thereby, ACE inhibitory peptide IFPPKPKDTL from porcine plasma was stable and has potential functional value.

Published in Food Chemistry: Molecular Sciences

ISSN: 2666-5662 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/food-chemistry-molecular-sciences

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