The R2R3 MYB Transcription Factor MYB71 Regulates Abscisic Acid Response in Arabidopsis

Yuxin Cheng; Yanxing Ma; Na Zhang; Rao Lin; Yuan Yuan; Hainan Tian; Saddam Hussain; Siyu Chen; Wenting Yang; Ling Cai; Yingying Li; Xiaoping Wang; Tianya Wang; Shucai Wang

doi:10.3390/plants11101369

Plants (May 2022)

The R2R3 MYB Transcription Factor MYB71 Regulates Abscisic Acid Response in Arabidopsis

Yuxin Cheng,
Yanxing Ma,
Na Zhang,
Rao Lin,
Yuan Yuan,
Hainan Tian,
Saddam Hussain,
Siyu Chen,
Wenting Yang,
Ling Cai,
Yingying Li,
Xiaoping Wang,
Tianya Wang,
Shucai Wang

Affiliations

Yuxin Cheng: Key Laboratory of Molecular Epigenetics of MOE, Northeast Normal University, Changchun 130024, China
Yanxing Ma: Key Laboratory of Molecular Epigenetics of MOE, Northeast Normal University, Changchun 130024, China
Na Zhang: Key Laboratory of Molecular Epigenetics of MOE, Northeast Normal University, Changchun 130024, China
Rao Lin: Key Laboratory of Molecular Epigenetics of MOE, Northeast Normal University, Changchun 130024, China
Yuan Yuan: Key Laboratory of Molecular Epigenetics of MOE, Northeast Normal University, Changchun 130024, China
Hainan Tian: Key Laboratory of Molecular Epigenetics of MOE, Northeast Normal University, Changchun 130024, China
Saddam Hussain: Key Laboratory of Molecular Epigenetics of MOE, Northeast Normal University, Changchun 130024, China
Siyu Chen: Laboratory of Plant Molecular Genetics & Crop Gene Editing, School of Life Sciences, Linyi University, Linyi 276000, China
Wenting Yang: Key Laboratory of Molecular Epigenetics of MOE, Northeast Normal University, Changchun 130024, China
Ling Cai: Key Laboratory of Molecular Epigenetics of MOE, Northeast Normal University, Changchun 130024, China
Yingying Li: Key Laboratory of Molecular Epigenetics of MOE, Northeast Normal University, Changchun 130024, China
Xiaoping Wang: Laboratory of Plant Molecular Genetics & Crop Gene Editing, School of Life Sciences, Linyi University, Linyi 276000, China
Tianya Wang: Key Laboratory of Molecular Epigenetics of MOE, Northeast Normal University, Changchun 130024, China
Shucai Wang: Key Laboratory of Molecular Epigenetics of MOE, Northeast Normal University, Changchun 130024, China

DOI: https://doi.org/10.3390/plants11101369
Journal volume & issue: Vol. 11, no. 10
p. 1369

Abstract

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Abscisic acid (ABA) regulates plant responses to abiotic stresses via regulating the expression of downstream genes, yet the functions of many ABA responsive genes remain unknown. We report here the characterization of MYB71, a R2R3 MYB transcription factor in regulating ABA responses in Arabidopsis. RT-PCR results show that the expression level of MYB71 was increased in response to ABA treatment. Arabidopsis protoplasts transfection results show that MYB71 was specifically localized in nucleus and it activated the Gal4:GUS reporter gene when recruited to the Gal4 promoter by a fused DNA binding domain GD. Roles of MYB71 in regulating plant response to ABA were analyzed by generating Arabidopsis transgenic plants overexpression MYB71 and gene edited mutants of MYB71. The results show that ABA sensitivity was increased in the transgenic plants overexpression MYB71, but decreased in the MYB71 mutants. By using a DEX inducible system, we further identified genes are likely regulated by MYB71, and found that they are enriched in biological process to environmental stimuli including abiotic stresses, suggesting that MYB71 may regulate plant response to abiotic stresses. Taken together, our results suggest that MYB71 is an ABA responsive gene, and MYB71 functions as a transcription activator and it positively regulates ABA response in Arabidopsis.

Published in Plants

ISSN: 2223-7747 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Botany
Website: http://www.mdpi.com/journal/plants

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