Nature Communications (Nov 2021)
Helicobacter pylori FabX contains a [4Fe-4S] cluster essential for unsaturated fatty acid synthesis
- Jiashen Zhou,
- Lin Zhang,
- Liping Zeng,
- Lu Yu,
- Yuanyuan Duan,
- Siqi Shen,
- Jingyan Hu,
- Pan Zhang,
- Wenyan Song,
- Xiaoxue Ruan,
- Jing Jiang,
- Yinan Zhang,
- Lu Zhou,
- Jia Jia,
- Xudong Hang,
- Changlin Tian,
- Houwen Lin,
- Hong-Zhuan Chen,
- John E. Cronan,
- Hongkai Bi,
- Liang Zhang
Affiliations
- Jiashen Zhou
- Department of Pharmacology and Chemical Biology, State Key Laboratory of Oncogenes and Related Genes, Shanghai Jiao Tong University School of Medicine
- Lin Zhang
- Department of Pharmacology and Chemical Biology, State Key Laboratory of Oncogenes and Related Genes, Shanghai Jiao Tong University School of Medicine
- Liping Zeng
- Department of Pathogen Biology & Jiangsu Key Laboratory of Pathogen Biology & Sir Run Run Hospital, Nanjing Medical University
- Lu Yu
- High Magnetic Field Laboratory, Chinese Academy of Sciences
- Yuanyuan Duan
- The Laboratory Center for Basic Medical Sciences, Nanjing Medical University
- Siqi Shen
- Department of Pharmacology and Chemical Biology, State Key Laboratory of Oncogenes and Related Genes, Shanghai Jiao Tong University School of Medicine
- Jingyan Hu
- Department of Pharmacology and Chemical Biology, State Key Laboratory of Oncogenes and Related Genes, Shanghai Jiao Tong University School of Medicine
- Pan Zhang
- Key Laboratory of Medical Epigenetics and Metabolism, Institutes of Biomedical Sciences, Shanghai Medical College, Fudan University
- Wenyan Song
- Department of Pharmacology and Chemical Biology, State Key Laboratory of Oncogenes and Related Genes, Shanghai Jiao Tong University School of Medicine
- Xiaoxue Ruan
- Department of Medicinal Chemistry, School of Pharmacy, Fudan University
- Jing Jiang
- Jiangsu Key Laboratory for Functional Substance of Chinese Medicine, School of Pharmacy, Nanjing University of Chinese Medicine
- Yinan Zhang
- Jiangsu Key Laboratory for Functional Substance of Chinese Medicine, School of Pharmacy, Nanjing University of Chinese Medicine
- Lu Zhou
- Department of Medicinal Chemistry, School of Pharmacy, Fudan University
- Jia Jia
- Department of Pathogen Biology & Jiangsu Key Laboratory of Pathogen Biology & Sir Run Run Hospital, Nanjing Medical University
- Xudong Hang
- Department of Pathogen Biology & Jiangsu Key Laboratory of Pathogen Biology & Sir Run Run Hospital, Nanjing Medical University
- Changlin Tian
- High Magnetic Field Laboratory, Chinese Academy of Sciences
- Houwen Lin
- Department of Pharmacology and Chemical Biology, State Key Laboratory of Oncogenes and Related Genes, Shanghai Jiao Tong University School of Medicine
- Hong-Zhuan Chen
- Institute of Interdisciplinary Integrative Biomedical Research, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine
- John E. Cronan
- Departments of Microbiology and Biochemistry, University of Illinois
- Hongkai Bi
- Department of Pathogen Biology & Jiangsu Key Laboratory of Pathogen Biology & Sir Run Run Hospital, Nanjing Medical University
- Liang Zhang
- Department of Pharmacology and Chemical Biology, State Key Laboratory of Oncogenes and Related Genes, Shanghai Jiao Tong University School of Medicine
- DOI
- https://doi.org/10.1038/s41467-021-27148-0
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 13
Abstract
Helicobacter pylori FabX, a dehydrogenase/isomerase flavoprotein, is required for unsaturated fatty acid synthesis. Here, the authors characterize FabX substrate recognition and catalytic mechanism, and reveal that it contains an atypical [4Fe-4S] cluster, which is essential and participates in the catalytic cycle.
