The social versus food preference test: A behavioral paradigm for studying competing motivated behaviors in rodents

Abstract

Behavior is influenced by a combination of factors, with the expression of the appropriate behavior dependent on an individual's current motivational state and the presence of stimuli in their surrounding environment. Thus far, most laboratory studies have focused on uncovering the peripheral and central systems that regulate the expression of a single behavior or the expression of a suite of behaviors associated with a single motivational state. In natural settings, however, an individual can be simultaneously experiencing multiple motivational states with multiple choices of how to act. Yet, the direct assessment of the roles of peripheral and central systems in coordinating motivated behavioral choice is largely understudied. This may be due to a lack of behavioral tests that are suitable for such investigations. Here, we describe a recently developed behavioral paradigm, hereafter called the Social versus Food Preference Test. This behavioral paradigm was validated in both rats and mice and is highly flexible, which will allow addressing of a wide range of research questions concerning the peripheral and central systems that coordinate the choice to seek social interaction versus the choice to seek food. • This paradigm was validated in rats and mice, the two most commonly used nonhuman species in behavioral research, but could be adapted for use in other rodent models. • The specific social and food stimuli used can be selected based on the research question. • Three-chamber apparatuses can be custom-constructed.

Keywords

• Investigation
• Social interaction-seeking
• Food-seeking
• Three-chamber test