Nature Communications (Jan 2021)
Structural basis for the multi-activity factor Rad5 in replication stress tolerance
- Miaomiao Shen,
- Nalini Dhingra,
- Quan Wang,
- Chen Cheng,
- Songbiao Zhu,
- Xiaolin Tian,
- Jun Yu,
- Xiaoxin Gong,
- Xuzhichao Li,
- Hongwei Zhang,
- Xin Xu,
- Liting Zhai,
- Min Xie,
- Ying Gao,
- Haiteng Deng,
- Yongning He,
- Hengyao Niu,
- Xiaolan Zhao,
- Song Xiang
Affiliations
- Miaomiao Shen
- Department of Biochemistry and Molecular Biology, Tianjin Medical University
- Nalini Dhingra
- Molecular Biology Department, Memorial Sloan Kettering Cancer Center
- Quan Wang
- Department of Molecular and Cellular Biochemistry, Indiana University Bloomington
- Chen Cheng
- State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry and Cell Biology, CAS Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences
- Songbiao Zhu
- MOE Key Laboratory of Bioinformatics, Center for Synthetic and Systematic Biology, School of Life Sciences, Tsinghua University
- Xiaolin Tian
- MOE Key Laboratory of Bioinformatics, Center for Synthetic and Systematic Biology, School of Life Sciences, Tsinghua University
- Jun Yu
- CAS Key Laboratory of Nutrition, Metabolism and Food safety, Shanghai Institute of Nutrition and Health, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
- Xiaoxin Gong
- Department of Biochemistry and Molecular Biology, Tianjin Medical University
- Xuzhichao Li
- Department of Biochemistry and Molecular Biology, Tianjin Medical University
- Hongwei Zhang
- CAS Key Laboratory of Nutrition, Metabolism and Food safety, Shanghai Institute of Nutrition and Health, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
- Xin Xu
- Department of Biochemistry and Molecular Biology, Tianjin Medical University
- Liting Zhai
- CAS Key Laboratory of Nutrition, Metabolism and Food safety, Shanghai Institute of Nutrition and Health, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
- Min Xie
- CAS Key Laboratory of Nutrition, Metabolism and Food safety, Shanghai Institute of Nutrition and Health, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
- Ying Gao
- CAS Key Laboratory of Nutrition, Metabolism and Food safety, Shanghai Institute of Nutrition and Health, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
- Haiteng Deng
- MOE Key Laboratory of Bioinformatics, Center for Synthetic and Systematic Biology, School of Life Sciences, Tsinghua University
- Yongning He
- State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry and Cell Biology, CAS Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences
- Hengyao Niu
- Department of Molecular and Cellular Biochemistry, Indiana University Bloomington
- Xiaolan Zhao
- Molecular Biology Department, Memorial Sloan Kettering Cancer Center
- Song Xiang
- Department of Biochemistry and Molecular Biology, Tianjin Medical University
- DOI
- https://doi.org/10.1038/s41467-020-20538-w
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 12
Abstract
Rad5 is a hub connecting three replication stress tolerance pathways. Here, the authors present the 3.3 Å crystal structure of a N-terminal truncated K.lactis Rad5 construct that reveals the spatial arrangement of the HIRAN, Snf2 and RING domains and structure-guided in vitro and in vivo experiments reveal multiple activities of the yeast Rad5 HIRAN domain among them a role in binding PCNA and supporting its ubiquitination.
