OncoTargets and Therapy (Oct 2020)
Knockdown of lncRNA PCAT1 Enhances Radiosensitivity of Cervical Cancer by Regulating miR-128/GOLM1 Axis
Abstract
Xingping Ge,1 Yongfei Gu,1 Dianzu Li,1 Maozhu Jiang,1 Shuliang Zhao,1 Zhengliang Li,1 Shuliang Liu2 1Department of Radiotherapy, Yantaishan Hospital, Yantai City, Shandong Province 264000, People’s Republic ofChina; 2Department of Thoracic Surgery, Yantaishan Hospital, Yantai City, Shandong Province 264001, People’s Republic of ChinaCorrespondence: Shuliang Liu Department of Thoracic SurgeryYantaishan Hospital, No. 91, Jiefang Road, Zhifu District, Yantai City, Shandong Province 26400, People’s Republic of ChinaTel +86-15863831132Email [email protected]: Cervical cancer (CC) is the fourth most common cancer with high death rate in females. The study aims to detect the mechanism of long non-coding RNA (LncRNA) PCAT1 on radiosensitivity of CC.Methods: The expression of PCAT1, miR-128 and GOLM1 in CC tissues and cells was measured by qRT-PCR. Different doses of X-ray were used for radiation treatment of CC cells and 6 Gy was chosen to perform the following experiments. The proliferation, migration and invasion of CC cells were measured by MTT assay, wound healing assay and transwell assay, respectively. The target relationships among PCAT1, miR-128 and GOLM1 were predicted by StarBase and TargetScan and verified by luciferase reporter assay. The protein level of GOLM1 was determined by Western blot. The xenograft tumor model was constructed in nude mice to verify the effect of PCAT1 on radiosensitivity of CC in vivo.Results: The PCAT1 expression was upregulated in CC tissues and cells. PCAT1 silencing enhances radiosensitivity of CC cells on proliferation, migration and invasion. MiR-128 was the target of PCAT1 and was negatively regulated by PCAT1. Upregulation of miR-128 enhances radiosensitivity of CC cells on proliferation, migration and invasion. GOLM1 was a target of miR-128 and was negatively regulated by miR-128. Upregulation of GOLM1 and downregulation of miR-128 both reversed the enhanced effect of PCAT1 knockdown on radiosensitivity of CC cells, which partly promoted the proliferation, migration and invasion of CC cells.Conclusion: Silencing of PCAT1 enhanced radiosensitivity of CC via targeting miR-128/GOLM1, which provided a new idea for treating CC.Keywords: cervical cancer, lncPCAT1, miR-128, GOLM1, radiosensitivity
