Constructing Optical Biosensor Based on Multienzyme System Tyrosinase/Horseradish Peroxidase

Abstract

By using sol-gel method, hybrid membranes were synthesized based on SiO2 and cellulose derivatives. Membranes showed good physical and mechanical properties as well as good biocompatibility. Onto hybrid membranes tyrosinase and horse radish peroxidase were immobilized and optical biosensor was constructed. The working principle of constructed biosensor was detection of oxygen consumption. The properties of constructed biosensors like linear range, life time and sensitivity were defined by modeling substrate - L-3,4-dihydroxyphenylalanine. The factors affecting the constructed optical biosensors were investigated. The results showed that a multienzyme system is capable of improving the constructed biosensor properties.

Keywords

• Optical biosensors
• Enzymes
• Sol-gel
• Toxic compounds detection