Nature Communications (May 2021)
Structure-based engineering of substrate specificity for pinoresinol-lariciresinol reductases
- Ying Xiao,
- Kai Shao,
- Jingwen Zhou,
- Lian Wang,
- Xueqi Ma,
- Di Wu,
- Yingbo Yang,
- Junfeng Chen,
- Jingxian Feng,
- Shi Qiu,
- Zongyou Lv,
- Lei Zhang,
- Peng Zhang,
- Wansheng Chen
Affiliations
- Ying Xiao
- Research and Development Center of Chinese Medicine Resources and Biotechnology, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine
- Kai Shao
- National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences
- Jingwen Zhou
- National Engineering Laboratory for Cereal Fermentation Technology and School of Biotechnology, Jiangnan University
- Lian Wang
- National Engineering Laboratory for Cereal Fermentation Technology and School of Biotechnology, Jiangnan University
- Xueqi Ma
- Research and Development Center of Chinese Medicine Resources and Biotechnology, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine
- Di Wu
- National Engineering Laboratory for Cereal Fermentation Technology and School of Biotechnology, Jiangnan University
- Yingbo Yang
- Research and Development Center of Chinese Medicine Resources and Biotechnology, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine
- Junfeng Chen
- Research and Development Center of Chinese Medicine Resources and Biotechnology, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine
- Jingxian Feng
- Research and Development Center of Chinese Medicine Resources and Biotechnology, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine
- Shi Qiu
- Research and Development Center of Chinese Medicine Resources and Biotechnology, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine
- Zongyou Lv
- Research and Development Center of Chinese Medicine Resources and Biotechnology, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine
- Lei Zhang
- Department of Pharmaceutical Botany, School of Pharmacy, Naval Medical University (Second Military Medical University)
- Peng Zhang
- National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences
- Wansheng Chen
- Research and Development Center of Chinese Medicine Resources and Biotechnology, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine
- DOI
- https://doi.org/10.1038/s41467-021-23095-y
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 11
Abstract
Pinoresinol–lariciresinol reductases (PLRs) are enzymes involved in the lignan biosynthesis. Here, crystal structures of three PLRs in the apo, substrate-bound and product-bound states, and accompanying mutagenesis provide insight into PLRs catalytic mechanism and suggest a strategy for PLR engineering.
