ACETIC ACID BACTERIA DETECTION IN WINES BY REAL-TIME PCR
DAN ZGARDAN,
IRINA MITINA,
VALENTIN MITIN,
EMILIA BEHTA,
SILVIA RUBTOV,
ALINA BOISTEAN,
RODICA STURZA,
MARIA MUNTEANU
Affiliations
DAN ZGARDAN
Technical University of Moldova, Faculty of Food Technology, Department of Oenology and Chemistry, 168, Stefan cel Mare Str., Chisinau MD-2004, Republic of Moldova
IRINA MITINA
The Institute of Genetics, Physiology and Plant Protection, 20, Padurilor Str., Chisinau MD-2002, Republic of Moldova
VALENTIN MITIN
The Institute of Genetics, Physiology and Plant Protection, 20, Padurilor Str., Chisinau MD-2002, Republic of Moldova
EMILIA BEHTA
Technical University of Moldova, Faculty of Food Technology, Department of Oenology and Chemistry, 168, Stefan cel Mare Str., Chisinau MD-2004, Republic of Moldova
SILVIA RUBTOV
Technical University of Moldova, Faculty of Food Technology, Department of Oenology and Chemistry, 168, Stefan cel Mare Str., Chisinau MD-2004, Republic of Moldova
ALINA BOISTEAN
Technical University of Moldova, Faculty of Food Technology, Department of Oenology and Chemistry, 168, Stefan cel Mare Str., Chisinau MD-2004, Republic of Moldova
RODICA STURZA
Technical University of Moldova, Faculty of Food Technology, Department of Oenology and Chemistry, 168, Stefan cel Mare Str., Chisinau MD-2004, Republic of Moldova
MARIA MUNTEANU
Technical University of Moldova, Faculty of Food Technology, Department of Oenology and Chemistry, 168, Stefan cel Mare Str., Chisinau MD-2004, Republic of Moldova
Acetic acid bacteria (AAB) are considered one of the most common wine spoilage microorganisms. They are still difficult to cultivate on laboratory media, which highlights the importance of alternative methods of detection of these bacteria. The goal of this work was development and testing of a fast and reliable Real Time Polymerase Chain Reaction (RT-PCR)-based method for easy detection of AAB in wine. We designed two primer sets for RT-PCR for detection of AAB and compared the results obtained using these primers with those obtained using a commercial kit. The results obtained using home designed primers showed good correlation with the results, obtained with the commercial screening kit.
