Molecular Fingerprinting Analysis of Yeasts Isolated from Traditional Guizhou Xiaoqu, a Traditional Fermentation Starter

Abstract

A total of 59 yeast isolates from Xiaoqu from five different regions of Guizhou were identified to belong to six species by sequencing of the D1/D2 domain of the 26S rRNA gene, including Trichosporon asahii (one strain), Saccharomycopsis fibuligera (32 strains), Saccharomycopsis malanga (8 strains), Hyphopichia burtonii (4 strains), Wickerhamomyces anomalus (6 strains), and Saccharomyces cerevisiae (8 strains). The colony characteristics of the six yeast species on WL medium and the restriction fragment length polymorphism patterns of the internal transcribed spacer (ITS) region of the 5.8S rRNA gene of these species were explored in this study in order to provide a basis for their fast identification. Tandem repeat-tRNA (TRtRNA) fingerprinting analysis was applied to discriminate intraspecific diversity, and 17 genotypes were found. Four, three, and eight different genotypes were identified from H. burtonii, W. anomalus, and S. fibuligera, respectively. The genotypes 10, 11, 12, 15, 16 and 17 of S. fibuligera had a close genetic relation, and the other genotypes from H. burtonii, W. anomalus, and S. fibuligera showed a relative far genetic relation at the intraspecific level.

Keywords

• yeast; restriction fragment length polymorphism analysis of the its region of 5.8s rrna gene; tandem repeat-trna fingerprinting method; genotype; cluster analysis