Supplementation of 5,6-Dimethylbenzimidazole and Cobalt in High-Concentrate Diet Improves the Ruminal Vitamin B<sub>12</sub> Synthesis and Fermentation of Sheep
Rui Zhang,
Zhiqiang Cheng,
Changjiang Zang,
Changyun Cui,
Changwen Zhang,
Yiling Jiao,
Fengming Li,
Xiaobin Li,
Kailun Yang,
Qiujiang Luo
Affiliations
Rui Zhang
Xinjiang Laboratory of Herbivore Nutrition for Meat and Milk, College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China
Zhiqiang Cheng
Xinjiang Laboratory of Herbivore Nutrition for Meat and Milk, College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China
Changjiang Zang
Xinjiang Laboratory of Herbivore Nutrition for Meat and Milk, College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China
Changyun Cui
Xinjiang Laboratory of Herbivore Nutrition for Meat and Milk, College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China
Changwen Zhang
Xinjiang Laboratory of Herbivore Nutrition for Meat and Milk, College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China
Yiling Jiao
Xinjiang Laboratory of Herbivore Nutrition for Meat and Milk, College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China
Fengming Li
Xinjiang Laboratory of Herbivore Nutrition for Meat and Milk, College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China
Xiaobin Li
Xinjiang Laboratory of Herbivore Nutrition for Meat and Milk, College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China
Kailun Yang
Xinjiang Laboratory of Herbivore Nutrition for Meat and Milk, College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China
Qiujiang Luo
Xinjiang Laboratory of Herbivore Nutrition for Meat and Milk, College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China
The objective of this experiment was to investigate the effects of 5,6-dimethylbenzimidazole (5,6-DMB) and cobalt (Co) on the ruminal vitamin B12 synthesis and fermentation parameters of sheep under high concentrate conditions. Twenty-four Kazakh rams (body weight = 39.23 ± 2.61 kg and 8 months old) fitted with permanent ruminal fistulas were randomly divided into four groups with six rams in each group. The control (CON) group was fed a basal ration with a concentrate-to-roughage ratio of 70:30, and the experimental groups (T60, T75 and T90) were fed a basal diet with 60 mg 5,6-DMB+ 0.25 mg Co, 75 mg 5,6-DMB+ 0.5 mg Co and 90 mg 5,6-DMB+ 0.75 mg Co supplied to each kilogram of basal diet, respectively. The experiment lasted for 26 days, with the first 14 days being an adaptation period to allow the sheep to adapt to the diet type and environment, and the second 12 days being a sample period. On 0, 7 and 12 d of the sample period, all sheep were weighed before the morning feed. Rumen fluid samples were collected from all sheep on the last 4 days of the sample period. The results showed that the ruminal vitamin B12 content was higher in trial groups than that of the CON group at 3 h after feeding (p p p p p 12, propionate, ammonia-N and microbial protein contents and pH in the rumen of sheep, and the best results were obtained by the amounts of 75 mg/kg 5,6-DMB and 0.5 mg/kg Co, respectively.
