Cytoprotective effect and purification of novel antioxidant peptides from hazelnut (C. heterophylla Fisch) protein hydrolysates

Chunlei Liu; Dayong Ren; Jingjing Li; Li Fang; Ji Wang; Jingsheng Liu; Weihong Min

Journal of Functional Foods (Mar 2018)

Cytoprotective effect and purification of novel antioxidant peptides from hazelnut (C. heterophylla Fisch) protein hydrolysates

Chunlei Liu,
Dayong Ren,
Jingjing Li,
Li Fang,
Ji Wang,
Jingsheng Liu,
Weihong Min

Affiliations

Chunlei Liu: College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, PR China; National Engineering Laboratory of Wheat and Corn Deep Processing, Changchun 130118, PR China
Dayong Ren: College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, PR China; National Engineering Laboratory of Wheat and Corn Deep Processing, Changchun 130118, PR China
Jingjing Li: College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, PR China; National Engineering Laboratory of Wheat and Corn Deep Processing, Changchun 130118, PR China
Li Fang: College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, PR China; National Engineering Laboratory of Wheat and Corn Deep Processing, Changchun 130118, PR China
Ji Wang: College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, PR China; National Engineering Laboratory of Wheat and Corn Deep Processing, Changchun 130118, PR China
Jingsheng Liu: College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, PR China; National Engineering Laboratory of Wheat and Corn Deep Processing, Changchun 130118, PR China
Weihong Min: Corresponding author at: No. 2888 Xincheng Street, College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, PR China.; College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, PR China; National Engineering Laboratory of Wheat and Corn Deep Processing, Changchun 130118, PR China

Journal volume & issue: Vol. 42
pp. 203 – 215

Abstract

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In the present study, antioxidant fraction (C2) has been obtained from a hazelnut processing by-product. The C2 exhibited strong antioxidant and cytoprotective effect against Ang II induced oxidant injury via up-regulation of SOD and heme oxygenase-1 (HO-1) levels and down-regulation of xanthine oxidase-1 (XO-1) level to inhibit the production of ROS synthesis in human umbilical vein endothelial cells (HUVECs). LC-ESI-MS/MS results revealed that C2 was found to include six novel peptides and their amino acid sequences were ADGF (408.16 Da), AGGF (350.16 Da), AWDPE (616.25 Da), DWDPK (659.29 Da), ETTL (462.23 Da), and SGAF (380.17 Da). All six synthetic peptides showed excellent antioxidant activity. Especially, the ADGF peptide exhibited the highest ABTS radical scavenging activity and DPPH radical scavenging ability among them. Therefore, the peptides isolated from wild hazelnut protein hydrolysates may be used as potential antioxidants in the food and drug industries.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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