Isolation and Characterization of Lignocellulolytic Bacteria from Municipal Solid Waste Landfill for Identification of Potential Hydrolytic Enzyme

Abstract

The utilization of lignocellulose biomass as an alternative source of renewable energy production via green technology is becoming important, and is in line with sustainable development goal initiatives. Lignocellulolytic bacteria, such as Bacillus spp., can break down biomass by producing hydrolytic enzymes, which are crucial in the successful conversion of biomass or lignocellulosic material into renewable energy. This information gave rise to this study, where municipal solid waste sediments of a sanitary municipal solid waste landfill were sampled and screened, and lignocellulolytic bacteria were isolated and characterized. Samples were taken from four different locations at the Pulau Burung landfill site in Malaysia. Lignin and starch were used as sources of carbon to identify potential bacteria that exhibit multi-enzymatic activity. The growth rate and doubling time of bacterial isolates in lignin and starch were taken as the criteria for selection. Eleven bacterial isolates were screened for cellulase activity using iodine and Congo red dyes. The cellulase activity of these isolates ranged from 0.8 to 1.7 U/mL. We carried out 16S rRNA gene sequencing to identify the phyla of the selected bacterial isolates. Phylogenetic analysis was also conducted based on the 16S rRNA sequences of the bacterial isolates and related Bacillus species, and a tree was generated using the Neighbor-Joining method. In this study, Bacillus proteolyticus, Bacillus Sanguinis, Bacillus spizizenii, Bacillus paramycoides, Bacillus paranthracis and Neobacillus fumarioli were identified as promising bacteria capable of expressing lignocellulolytic enzymes and degrading the lignocellulosic biomass present in municipal solid waste.

Keywords

• <i>Bacillus</i> spp
• cellulase
• lignocellulose biomass
• lignocellulolytic bacteria
• municipal solid waste