Journal of Functional Foods (Jun 2016)

γ-Glutamyl valine supplementation-induced mitigation of gut inflammation in a porcine model of colitis

  • Hua Zhang,
  • Tomohiro Kodera,
  • Yuzuru Eto,
  • Yoshinori Mine

Journal volume & issue
Vol. 24
pp. 558 – 567

Abstract

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A dietary peptide γ-glutamyl valine (EV) possesses the agonistic property to activate a calcium-sensing receptor-mediated anti-inflammatory pathway, thereby suppressing inflammation. This study aims to substantiate the therapeutic efficacy of γ-EV as an anti-inflammatory agent in a dextran sodium sulphate (DSS)-induced colitis porcine model. Colitis symptoms were observed after intra-gastric infusion of DSS for five days, followed by five days of administration of saline or γ-EV. γ-EV supplementation reduced DSS-induced weight loss and clinical signs, while preserving colonic architecture and intestinal permeability. Colonic production of tumour necrosis factor (TNF)-α and interleukin (IL)-6 were reduced to a level observed in animals without DSS treatment. The colonic gene expression of TNF-α, IL-6, IL-1β and interferon (IFN)-γ were also mitigated by γ-EV treatment, thus indicating γ-EV can restore immune homeostasis by attenuating inflammation. Our findings suggest that γ-EV supplementation may be a promising therapeutic strategy in the treatment of intestinal inflammation.

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