Analysis of enniatins and beauvericin by LC-MS/MS in wheat-based products

Abstract

Due to the matrix complexity for wheat-based products, a comparative study of different rapid extraction procedures was performed for the extraction of enniatins (ENA, ENA1, ENB, ENB1) and beauvericin in flour, pasta, breakfast cereals, and biscuits. Three different approaches were studied during the extraction and purification steps (shaker, Ultra-Turrax, and QuEChERS) for each matrix. Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) with electrospray source working in a positive mode was used. For the analysis of the five mycotoxins, the three methods were tested in terms of recovery, matrix effect, and sensibility, concluding that Ultra-Turrax extraction was the most competent method. The applicability of the validated method was demonstrated by analyzing 16 commercial samples from Romania.

Keywords

• Emerging mycotoxins
• LC-MS/MS
• method validation
• cereal-based products