Serine prevents LPS-induced intestinal inflammation and barrier damage via p53-dependent glutathione synthesis and AMPK activation

Xihong Zhou; Yumei Zhang; Liuqin He; Dan Wan; Gang Liu; Xin Wu; Yulong Yin

Journal of Functional Foods (Dec 2017)

Serine prevents LPS-induced intestinal inflammation and barrier damage via p53-dependent glutathione synthesis and AMPK activation

Xihong Zhou,
Yumei Zhang,
Liuqin He,
Dan Wan,
Gang Liu,
Xin Wu,
Yulong Yin

Affiliations

Xihong Zhou: Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China; National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Changsha 410125, China; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Changsha 410125, China; Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha 410125, China; State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing 100193, China
Yumei Zhang: Hunan Co-Innovation Center of Animal Production Safety, CICAPS, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China
Liuqin He: Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China; National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Changsha 410125, China; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Changsha 410125, China; Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha 410125, China
Dan Wan: Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China; National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Changsha 410125, China; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Changsha 410125, China; Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha 410125, China
Gang Liu: Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China; National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Changsha 410125, China; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Changsha 410125, China; Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha 410125, China; Taoyuan Agro-ecosystem Research Station, Soil Molecular Ecology Section, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China; Corresponding authors at: Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China.
Xin Wu: Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China; National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Changsha 410125, China; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Changsha 410125, China; Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha 410125, China; Corresponding authors at: Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China.
Yulong Yin: Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China; National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Changsha 410125, China; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Changsha 410125, China; Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha 410125, China; Corresponding authors at: Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China.

Journal volume & issue: Vol. 39
pp. 225 – 232

Abstract

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The role of serine in metabolic processes has recently attracted much attention as it could be used for the biosynthesis of purine, sphingolipids, and glutathione. The present study was conducted to investigate the effects of serine on LPS-induced intestinal inflammation and barrier dysfunction. The results showed that pretreatment with serine prevented the LPS-induced inflammatory response and oxidative stress both in vivo and in vitro. Moreover, serine maintained intestinal barrier function and permeability. In addition, we found that serine improved glutathione synthesis and AMP-activated protein kinase (AMPK) activity. However, following the small interfering RNA (siRNA) inhibition of p53 expression or inhibition of glutathione synthesis in IPEC-1 cells, the protective effects of serine on LPS-induced damage were diminished. Our results indicated that serine may prevent LPS-induced damage in the intestine by improving glutathione synthesis and AMPK activity in a p53-dependent manner.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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