Polyphenolic profile of persimmon leaves by high resolution mass spectrometry (LC-ESI-LTQ-Orbitrap-MS)

Abstract

Persimmon leaves have played an important role in Chinese medicine. Persimmon extracts and formulations have been shown to possess a wide range of pharmacological activities, including antioxidant, hypolipidaemic and antidiabetic, and they have been used to treat cardiovascular disease, improve homeostasis, as antibacterial and anti-inflammatory agents, and as a beauty treatment. In this work, liquid chromatography coupled to hybrid linear ion trap quadrupole Orbitrap mass spectrometry was used to accurately identify persimmon leaf polyphenols. Forty-one phenolic compounds, including simple phenolic acids, hydroxybenzoic acids, hydroxycinnamic acids, flavanols, flavonols, flavanones, flavone-chalcones, tyrosols and their conjugated derivatives, were identified and quantified using high mass accuracy data and confirmed by MS2 experiments. To the best of our knowledge, this is the most extensive study of persimmon leaf polyphenols performed so far, since 33 phenolic compounds are reported for the first time.

Keywords

• Persimmon leaves
• Orbitrap-MS
• Flavonoids
• Cinnamic acid
• Hydroxybenzoic acids
• Hydroxytyrosol