Lipid Recovery from Microalgae Biomass Using Sugaring-Out Extraction in Liquid Biphasic Flotation System
Nurul Syahirah Mat Aron,
Kit Wayne Chew,
Zengling Ma,
Yang Tao,
Malinee Sriariyanun,
Inn Shi Tan,
Cường Nguyễn Mạnh,
Ao Xia,
Tonni Agustiono Kurniawan,
Pau Loke Show
Affiliations
Nurul Syahirah Mat Aron
Zhejiang Provincial Key Laboratory for Subtropical Water Environment and Marine Biological Resources Protection, Wenzhou University, Wenzhou 325035, China
Kit Wayne Chew
Zhejiang Provincial Key Laboratory for Subtropical Water Environment and Marine Biological Resources Protection, Wenzhou University, Wenzhou 325035, China
Zengling Ma
Zhejiang Provincial Key Laboratory for Subtropical Water Environment and Marine Biological Resources Protection, Wenzhou University, Wenzhou 325035, China
Yang Tao
College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
Malinee Sriariyanun
Biorefinery and Process Automation Engineering Center, Department of Chemical and Process Engineering, The Sirindhorn International Thai-German Graduate School of Engineering, King Mongkut University of Technology North Bangkok, Bangkok 10800, Thailand
Inn Shi Tan
Department of Chemical and Energy Engineering, Curtin University, 98009 Miri, Sarawak, Malaysia
Cường Nguyễn Mạnh
Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Rd., Cau Giay, Hanoi 100915, Vietnam
Ao Xia
Key Laboratory of Low-Grade Energy Utilization Technologies and Systems, Chongqing University, Ministry of Education, Chongqing 400044, China
Tonni Agustiono Kurniawan
College of the Environment and Ecology, Xiamen University, Xiamen 361102, China
Pau Loke Show
Zhejiang Provincial Key Laboratory for Subtropical Water Environment and Marine Biological Resources Protection, Wenzhou University, Wenzhou 325035, China
The increase in global temperature calls for ambitious action to reduce the release of greenhouse gases into the atmosphere. The transportation sector contributes up to 25% of the total emissions released, mainly from the burning of vehicle fuel. Therefore, scientists from all around the world are focusing on finding a sustainable alternative to conventional vehicle fuel. Biofuel has attracted much attention, as it shows great potential for the replacement of traditional fossil fuels. However, the main bottlenecks of biofuel are the ongoing controversial conflict between food security with biofuel production. Therefore, this study focuses on a sustainable extraction of lipids from microalgae for the production of biofuel using a liquid biphasic flotation system coupled with sugaring-out method. This is the first study to combine the methods of liquid biphasic flotation system with the sugaring-out technique. It represents a holistic study of optimum and effective conditions needed to extract lipids from the system and to understand the reliability of sugar solution as the agent of cell disruption. At the 15-min flotation time, 150 g/L of fructose solution with a 1:2 mass separating agent-acetonitrile ratio successfully extracted up to 74% of lipid from Chlorella sorokiniana CY-1. Two types of fatty acid methyl esters were recovered from the study, with C5:0 being the main component extracted.
