Lactobacillus pentosus S-PT84 prevents LPS-induced low-grade chronic inflammation in a C57BL/6J mouse model

Abstract

Chronic exposure to low-dose of endotoxin triggers a pronounced inflammatory responses impairing gut physical barrier and resulting in microbiota dysbiosis. In this study, we investigated the anti-inflammatory activity of dietary heat killed (HK) Lactobacillus pentosus S-PT84 in a lipopolysaccharides (LPS)-induced low-grade chronic inflammation C57BL/6J mouse model. The results showed that the deficient expression of tight junction proteins (ZO-1, Cldn 3 and JAMA) and mucin 2 were restored by HK L. pentosus S-PT84. Subsequently, HK L. pentosus S-PT84 attenuated the secretion of pro-inflammatory mediators (TNF-α and MCP-1) and the decreased levels of adiponectin with a reduction in macrophage infiltration in white adipose tissue (WAT). Moreover, the dysfunctional metabolic activity observed in WAT was significantly inhibited through upregulation of PPAR-γ and IRS-1 expression. The dysbiosis in gut microbiota were prevented by HK L. pentosus S-PT84. These findings suggest that HK L. pentosus S-PT84 exerts protective effects on intestinal mucosal integrity and an early risk for progression into metabolic disorders.

Keywords

• Lactobacillus pentosus S-PT84
• Gut inflammation
• Low-grade chronic inflammation
• Metabolic disorder
• Gut microbiota