Protective effects of Bacillus amyloliquefaciens-derived nonapeptide in cyclophosphamide-induced immunosuppressed mice

Rong Yan; Xiaoxi Lu; Yalin Liu; Qifan Zhang; Fengqin Wang; Mingliang Jin; Haiqiao He; Yizhen Wang

Journal of Functional Foods (Aug 2024)

Protective effects of Bacillus amyloliquefaciens-derived nonapeptide in cyclophosphamide-induced immunosuppressed mice

Rong Yan,
Xiaoxi Lu,
Yalin Liu,
Qifan Zhang,
Fengqin Wang,
Mingliang Jin,
Haiqiao He,
Yizhen Wang

Affiliations

Rong Yan: Key Laboratory of Molecular Nutrition, Ministry of Education, Zhejiang University, Hangzhou 310058, China; Key Laboratory of Animal Nutrition and Feed, Ministry of Agriculture, Zhejiang University, Hangzhou 310058, China; Key Laboratory of Animal Nutrition and Feed Nutrition of Zhejiang Province, Zhejiang University, Hangzhou 310058, China; Institute of Feed Science, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China
Xiaoxi Lu: Key Laboratory of Molecular Nutrition, Ministry of Education, Zhejiang University, Hangzhou 310058, China; Key Laboratory of Animal Nutrition and Feed, Ministry of Agriculture, Zhejiang University, Hangzhou 310058, China; Key Laboratory of Animal Nutrition and Feed Nutrition of Zhejiang Province, Zhejiang University, Hangzhou 310058, China; Institute of Feed Science, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China
Yalin Liu: Key Laboratory of Molecular Nutrition, Ministry of Education, Zhejiang University, Hangzhou 310058, China; Key Laboratory of Animal Nutrition and Feed, Ministry of Agriculture, Zhejiang University, Hangzhou 310058, China; Key Laboratory of Animal Nutrition and Feed Nutrition of Zhejiang Province, Zhejiang University, Hangzhou 310058, China; Institute of Feed Science, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China
Qifan Zhang: Key Laboratory of Molecular Nutrition, Ministry of Education, Zhejiang University, Hangzhou 310058, China; Key Laboratory of Animal Nutrition and Feed, Ministry of Agriculture, Zhejiang University, Hangzhou 310058, China; Key Laboratory of Animal Nutrition and Feed Nutrition of Zhejiang Province, Zhejiang University, Hangzhou 310058, China; Institute of Feed Science, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China
Fengqin Wang: Key Laboratory of Molecular Nutrition, Ministry of Education, Zhejiang University, Hangzhou 310058, China; Key Laboratory of Animal Nutrition and Feed, Ministry of Agriculture, Zhejiang University, Hangzhou 310058, China; Key Laboratory of Animal Nutrition and Feed Nutrition of Zhejiang Province, Zhejiang University, Hangzhou 310058, China; Institute of Feed Science, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China
Mingliang Jin: Key Laboratory of Molecular Nutrition, Ministry of Education, Zhejiang University, Hangzhou 310058, China; Key Laboratory of Animal Nutrition and Feed, Ministry of Agriculture, Zhejiang University, Hangzhou 310058, China; Key Laboratory of Animal Nutrition and Feed Nutrition of Zhejiang Province, Zhejiang University, Hangzhou 310058, China; Institute of Feed Science, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China
Haiqiao He: Guangzhou Bestide Bio-Science and Technology Co., Ltd., Guangzhou 51000, China
Yizhen Wang: Key Laboratory of Molecular Nutrition, Ministry of Education, Zhejiang University, Hangzhou 310058, China; Key Laboratory of Animal Nutrition and Feed, Ministry of Agriculture, Zhejiang University, Hangzhou 310058, China; Key Laboratory of Animal Nutrition and Feed Nutrition of Zhejiang Province, Zhejiang University, Hangzhou 310058, China; Institute of Feed Science, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China; Corresponding authors at: Key Laboratory of Molecular Nutrition, Ministry of Education, Zhejiang University, Hangzhou 310058, China.

Journal volume & issue: Vol. 119
p. 106273

Abstract

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This study aims to explore the effect of Bacillus amyloliquefaciens-derived nonapeptide (BAP) on growth and immune function in cyclophosphamide (Cy)-induced immunosuppressed mice. The results showed that BAP alleviated the reduction of the body weight, daily feed intake and spleen and thymus index caused by Cy, and restored jejunal tissue morphology, goblet cell numbers and tight junction proteins. BAP enhanced the expression of immunoglobulins, including immunoglobulin M (IgM), immunoglobulin G (IgG) and secretory immunoglobulin A (SIgA), jejunal CD3+, CD4+ and CD8+ lymphocytes, and cytokines including interleukin-2 (IL-2) and interferon-γ (IFN-γ), while it inhibited the expression of interleukin-4 (IL-4) and interleukin-10 (IL-10). The oxidative stress and decreased antioxidant enzymes induced by Cy were also reversed by BAP. Moreover, BAP modulated the structure of gut microbiota and promoted the colonization of potential intestinal probiotics. Our findings establish the groundwork for comprehending the function of BAP and its potential utilization as an immunomodulator.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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