Chemomodulatory effects of Ichnocarpus frutescens R. Br against 4-vinylcyclohexane induced ovarian cancer in swiss albino mice

Abstract

Objective: To evaluate the protective effect of Ichnocarpus frutescens (I. frutescens) R. Br against 4-vinylcyclohexane induced ovarian cancer in Swiss albino mice. Methods: The ovarian cancer was induced by 4-vinylcyclohexane which was given to 28-day-old mice in corn oil, intra peritoneally, at 2.7 mmol/kg body weight/day for 30 d. After that serum and tissue were isolated. Enzymatic antioxidants, non-enzymatic antioxidants, lipid peroxidation assessed in liver and ovary homogenate. Metabolites, marker enzymes were analyzed in serum. Tissue was examined histopathology examination. Results: The levels of urea, creatinine, marker enzymes and lipid peroxidation were significantly increased in ovarian cancer induced mice when compared to control group whereas, levels of uric acid, enzymatic and non-enzymatic antioxidants were decreased in cancer induced mice when compared with control mice. From these parameters were brought back to near normal in ethanolic extract of I. frutescens treated animals. The above results were further confirmed by histopathological examination which shows marked edema of lamina propria occurred in cancer induced animals whereas no alterations in ethanolic extract of I. frutescens and cisplatin treated groups. Conclusions: This present study was evaluated that the ethanolic extracts of I. frutescens have effective anticancer activity against 4-vinylcyclohexane induced ovarian cancer.

Keywords

• 4-vinylcyclohexane
• Antioxidants
• Ethanolic extract
• Ichnocarpus frutescens