OPTIMIZATION OF HIBISCUS SABDARIFFA L. (ROSELLE) ANTHOCYANIN AQUEOUS-ETHANOL EXTRACTION PARAMETERS USING RESPONSE SURFACE METHODOLOGY
ANILÚ MIRANDA-MEDINA,
PATRICIA M. HAYWARD-JONES,
OCTAVIO CARVAJAL-ZARRABAL,
LUZ DEL ALBA LADRÓN DE GUEVARA-VELA,
YERIKC DAVID RAMÍREZ-VILLAGÓMEZ,
DULCE M. BARRADAS-DERMITZ,
GEORGINA LUNA-CARRILLO,
MARÍA G. AGUILAR-USCANGA
Affiliations
ANILÚ MIRANDA-MEDINA
Veracruz Institute of Technology, Chemical and Biochemical Engineering Department, Miguel Ángel de Quevedo 2779, Colonia Formando Hogar 91780, Veracruz, Mexico
PATRICIA M. HAYWARD-JONES
Veracruz Institute of Technology, Biological Chemistry Area, Miguel Ángel de Quevedo 2779, Colonia Formando Hogar 91780, Veracruz, Mexico
OCTAVIO CARVAJAL-ZARRABAL
University of Veracruz, Nutrition Chemistry and Biochemistry Area, Juan Pablo II s/n 94294, Boca del Río, Mexico
LUZ DEL ALBA LADRÓN DE GUEVARA-VELA
Veracruz Institute of Technology, Chemical and Biochemical Engineering Department, Miguel Ángel de Quevedo 2779, Colonia Formando Hogar 91780, Veracruz, Mexico
YERIKC DAVID RAMÍREZ-VILLAGÓMEZ
Veracruz Institute of Technology, Chemical and Biochemical Engineering Department, Miguel Ángel de Quevedo 2779, Colonia Formando Hogar 91780, Veracruz, Mexico
DULCE M. BARRADAS-DERMITZ
Veracruz Institute of Technology, Biological Chemistry Area, Miguel Ángel de Quevedo 2779, Colonia Formando Hogar 91780, Veracruz, Mexico
GEORGINA LUNA-CARRILLO
Veracruz Institute of Technology, Chemical and Biochemical Engineering Department, Miguel Ángel de Quevedo 2779, Colonia Formando Hogar 91780, Veracruz, Mexico
MARÍA G. AGUILAR-USCANGA
Veracruz Institute of Technology, Chemical and Biochemical Engineering Department, Miguel Ángel de Quevedo 2779, Colonia Formando Hogar 91780, Veracruz, Mexico
Anthocyanins along with protocatechuic acid and quercetin have been recognized as bioactive compounds in Hibiscus sabdariffa L. aqueous extracts. Characteristic anthocyanin absorption in the visible region makes their quantification possible without the interference of the other two compounds, and also can favor its potential application as an alternative to organic-based dye sensitized solar cell, in various forms. In order to optimize measurable factors linked to the extraction of these flavonoids, an optimization was performed using a Box-Behnken experimental design and response surface methodology (RSM). Three levels of ethanol concentration, temperature and solid-solvent ratio (SSR) were investigated. The optimization model showed that with 96 % EtOH, 65 °C, and 1:50 SSR, the highest anthocyanin concentration of 150 mg/100 g was obtained.
