Prowashonupana barley dietary fibre reduces body fat and increases insulin sensitivity in Caenorhabditis elegans model

Chenfei Gao; Michael L. King; Zachary L. Fitzpatrick; Wenqian Wei; Jason F. King; Mingming Wang; Frank L. Greenway; John W. Finley; Jeffrey H. Burton; William D. Johnson; Michael J. Keenan; Frederick M. Enright; Roy J. Martin; Jolene Zheng

Journal of Functional Foods (Oct 2015)

Prowashonupana barley dietary fibre reduces body fat and increases insulin sensitivity in Caenorhabditis elegans model

Chenfei Gao,
Michael L. King,
Zachary L. Fitzpatrick,
Wenqian Wei,
Jason F. King,
Mingming Wang,
Frank L. Greenway,
John W. Finley,
Jeffrey H. Burton,
William D. Johnson,
Michael J. Keenan,
Frederick M. Enright,
Roy J. Martin,
Jolene Zheng

Affiliations

Chenfei Gao: School of Nutrition and Food Sciences, College of Agriculture, Louisiana State University, Baton Rouge, LA, USA
Michael L. King: Department of Biological Science, College of Basic Science, Louisiana State University, Baton Rouge, LA, USA
Zachary L. Fitzpatrick: Department of Biological Science, College of Basic Science, Louisiana State University, Baton Rouge, LA, USA
Wenqian Wei: School of Life Sciences, Fudan University, Shanghai, China
Jason F. King: School of Nutrition and Food Sciences, College of Agriculture, Louisiana State University, Baton Rouge, LA, USA
Mingming Wang: School of Nutrition and Food Sciences, College of Agriculture, Louisiana State University, Baton Rouge, LA, USA
Frank L. Greenway: Pennington Biomedical Research Center, Louisiana State University, Baton Rouge, LA 70808, USA
John W. Finley: School of Nutrition and Food Sciences, College of Agriculture, Louisiana State University, Baton Rouge, LA, USA
Jeffrey H. Burton: Pennington Biomedical Research Center, Louisiana State University, Baton Rouge, LA 70808, USA
William D. Johnson: Pennington Biomedical Research Center, Louisiana State University, Baton Rouge, LA 70808, USA
Michael J. Keenan: School of Nutrition and Food Sciences, College of Agriculture, Louisiana State University, Baton Rouge, LA, USA; Pennington Biomedical Research Center, Louisiana State University, Baton Rouge, LA 70808, USA
Frederick M. Enright: School of Animal Sciences, College of Agriculture, Louisiana State University, Baton Rouge, LA, USA
Roy J. Martin: University of California at Davis, Davis, CA 95616, USA
Jolene Zheng: Pennington Biomedical Research Center, Louisiana State University, Baton Rouge, LA 70808, USA; Corresponding author. Pennington Biomedical Research Center, Louisiana State University, Baton Rouge, LA 70808, USA. Tel.: +1 225 763 2880; fax: +1 225 763 2847.

Journal volume & issue: Vol. 18
pp. 564 – 574

Abstract

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Prowashonupana barley (PWB) is high in β-glucan with moderate content of resistant starch. PWB reduced intestinal fat deposition (IFD) in wild type Caenorhabditis elegans (C. elegans, N2), and in sir-2.1 or daf-16 null mutants, and sustained a surrogate marker of lifespan, pharyngeal pumping rate (PPR), in N2, sir-2.1, daf-16, or daf-16/daf-2 mutants. Hyperglycaemia (2% glucose) reversed or reduced the PWB effect on IFD in N2 or daf-16/daf-2 mutants with a sustained PPR. mRNA expression of cpt-1, cpt-2, ckr-1, and gcy-8 were dose-dependently reduced in N2 or daf-16 mutants, elevated in daf-16/daf-2 mutants with reduction in cpt-1, and unchanged in sir-2.1 mutants. mRNA expressions were increased by hyperglycaemia in N2 or daf-16/daf-2 mutants, while reduced in sir-2.1 or daf-16 mutants. The effects of PWB in the C. elegans model appeared to be primarily mediated via sir-2.1, daf-16, and daf-16/daf-2. These data suggest that PWB and β-glucans may benefit hyperglycaemia-impaired lipid metabolism.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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