Study on Vanillin Loaded on Corn Bran Arabinoxylan Composite Nanocarrier and Its Antibacterial Property

Abstract

In order to improve the stability of vanillin by polysaccharide-protein interaction and to investigate the inhibitory effect of the composite nanocarrier on Listeria monocytogenes and E. coli, a vanillin-loaded composite nanocarrier of corn bran arabinoxylan (AX) and zein was prepared. Two crude polysaccharide fractions of corn bran were obtained by precipitating with 80% and 90% ethanol. Four fractions (F80-1, F80-2, F90-1, and F90-2) were subjected to anion-exchange chromatography purification. The monosaccharide composition of the fractions was determined using gas chromatography. It was found that the total content of arabinose and xylose in each fraction was 67.41%, 76.12%, 77.16%, and 84.89%, respectively, and the encapsulation rate of vanillin in the composite nanoparticles of the four fractions was 81.18%, 69.36%, 86.80%, and 88.48%, respectively. The vanillin encapsulation and the intermolecular interaction in the composite nanocarriers were verified by fluorescence spectrometry and differential scanning calorimetry (DSC). Bacterial inhibition experiments showed that F80-2 composite nanocarriers were most effective at inhibiting Listeria monocytogenes, and F90-2 composite nanocarriers were most effective at inhibiting E. coli.

Keywords

• corn bran arabinoxylan
• zein
• vanillin
• nanoparticles
• listeria monocytogenes
• e. coli
• bacteriostatic property