Purification and hypotensive activity of rapeseed protein-derived renin and angiotensin converting enzyme inhibitory peptides

Rong He; Sunday A. Malomo; Adeola Alashi; Abraham T. Girgih; Xingrong Ju; Rotimi E. Aluko

Journal of Functional Foods (Apr 2013)

Purification and hypotensive activity of rapeseed protein-derived renin and angiotensin converting enzyme inhibitory peptides

Rong He,
Sunday A. Malomo,
Adeola Alashi,
Abraham T. Girgih,
Xingrong Ju,
Rotimi E. Aluko

Affiliations

Rong He: College of Food Science, Jiangnan University, Wuxi 214122, People’s Republic of China; Department of Human Nutritional Sciences and the Richardson Centre for Functional Foods and Nutraceuticals, University of Manitoba, Winnipeg, Manitoba, Canada R3T 2N2
Sunday A. Malomo: Department of Human Nutritional Sciences and the Richardson Centre for Functional Foods and Nutraceuticals, University of Manitoba, Winnipeg, Manitoba, Canada R3T 2N2
Adeola Alashi: Department of Human Nutritional Sciences and the Richardson Centre for Functional Foods and Nutraceuticals, University of Manitoba, Winnipeg, Manitoba, Canada R3T 2N2; School of Agriculture and Wine Sciences, Charles Sturt University, PB 588, Wagga Wagga NSW, Australia
Abraham T. Girgih: Department of Human Nutritional Sciences and the Richardson Centre for Functional Foods and Nutraceuticals, University of Manitoba, Winnipeg, Manitoba, Canada R3T 2N2
Xingrong Ju: College of Food Science, Jiangnan University, Wuxi 214122, People’s Republic of China; College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210003, People’s Republic of China; Corresponding authors. Tel.: +1 204 474 9555; fax: +1 204 474 7593 (R.E. Aluko); tel.: +86 25 8402 8788; fax: +86 25 8402 8788 (X. Ju).
Rotimi E. Aluko: Department of Human Nutritional Sciences and the Richardson Centre for Functional Foods and Nutraceuticals, University of Manitoba, Winnipeg, Manitoba, Canada R3T 2N2; Corresponding authors. Tel.: +1 204 474 9555; fax: +1 204 474 7593 (R.E. Aluko); tel.: +86 25 8402 8788; fax: +86 25 8402 8788 (X. Ju).

Journal volume & issue: Vol. 5, no. 2
pp. 781 – 789

Abstract

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Rapeseed protein isolate (RPI) was hydrolyzed with Alcalase followed by reverse-phase high performance liquid chromatography (RP-HPLC) purification of bioactive peptides. The rapeseed protein hydrolysate (RPH) obtained after 4 h digestion with Alcalase had a degree of hydrolysis (DH) of ∼11%. Gel permeation chromatography separation showed high contents of low molecular weight peptides in the RPH when compared to the RPI. After preparative and analytical RP-HPLC separations, three peptides (LY, TF and RALP) were purified and amino acid sequence determined by tandem mass spectrometry. LY (IC50, 0.11 mM) was the most potent (p < 0.05) against ACE activity when compared to TF (IC50, 0.81 mM) and RALP (IC50, 0.65 mM). However, RALP (IC50, 0.97 mM) was the most potent (p < 0.05) against renin activity when compared to LY (IC50, 1.87 mM) and TF (IC50, 3.1 mM). Single oral administration (30 mg/kg body weight) to spontaneously hypertensive rats showed LY and RALP to be the more effective hypotensive agents with maximum blood pressure reduction of −26 and 16 mmHg, respectively when compared to TF (−12 mmHg). The results suggest that the higher number of hydrophobic amino acid residues LY and RALP contributed to their higher in vitro and in vivo activities when compared to TF.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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