EFSA Journal (Aug 2021)

Safety evaluation of steviol glycoside preparations, including rebaudioside AM, obtained by enzymatic bioconversion of highly purified stevioside and/or rebaudioside A stevia leaf extracts

  • EFSA Panel on Food Additives and Flavourings (FAF),
  • Maged Younes,
  • Gabriele Aquilina,
  • Laurence Castle,
  • Karl‐Heinz Engel,
  • Paul Fowler,
  • Maria Jose Frutos Fernandez,
  • Peter Fürst,
  • Rainer Gürtler,
  • Ursula Gundert‐Remy,
  • Trine Husøy,
  • Melania Manco,
  • Wim Mennes,
  • Sabina Passamonti,
  • Peter Moldeus,
  • Romina Shah,
  • Ine Waalkens‐Berendsen,
  • Detlef Wölfle,
  • Matthew Wright,
  • José Manuel Barat Baviera,
  • Gisela Degen,
  • Jean‐Charles Leblanc,
  • Lieve Herman,
  • Alessandra Giarola,
  • Jaime Aguilera,
  • Giorgia Vianello,
  • Laurence Castle

DOI
https://doi.org/10.2903/j.efsa.2021.6691
Journal volume & issue
Vol. 19, no. 8
pp. n/a – n/a

Abstract

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Abstract The EFSA Panel on Food Additives and Flavourings (FAF) provides a scientific opinion on the safety of steviol glycoside preparations, including rebaudioside AM, obtained by enzymatic bioconversion of highly purified stevioside and/or rebaudioside A stevia leaf extracts. These steviol glycoside preparations are produced via enzymatic bioconversion of highly purified stevioside and/or rebaudioside A extracts obtained from stevia plant using two UDP‐glucosyltransferases and one sucrose synthase enzymes produced by the genetically modified strains of E. coli K‐12 that facilitate the transfer of glucose to purified stevia leaf extracts via glycosidic bonds. The Panel considered that the parental strain is a derivative of E. coli K‐12 which is well characterised and its safety has been documented; therefore, it is considered to be safe for production purposes. The Panel concluded that there is no safety concern for steviol glycoside preparations, including rebaudioside AM, obtained by enzymatic bioconversion of highly purified stevioside and/or rebaudioside A stevia leaf extracts using UDP‐glucosyltransferases and sucrose synthase enzymes produced by the genetically modified strains of E. coli K‐12, to be used as a food additive. The Panel recommends the European Commission to consider the proposal of establishing separate specifications for steviol glycoside preparations, including rebaudioside AM, obtained by enzymatic bioconversion of highly purified stevioside and/or rebaudioside A stevia leaf extracts in Commission Regulation (EU) No 231/2012.

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