Development of quality control parameters for the standardization of gymnema sylvestre

Abstract

Objective: To develop a novel qualitative and quantitative technique, which can pave the way for rapid and selective determination of different phytoconstituents of Gymnema sylvestre (G. sylvestre). Methods: Phytochemical test, TLC analysis, solubility study, total phenol and flavonoid content and antimicrobial study were performed in the present investigation. Fingerprint analysis and quantitative analysis of quercetin were also performed through hptlc method. Results: Alkaloid, saponin, tannin, triterpenoid and flavonoid were found to be present in the G. sylvestre extract. Solubility in water and alcohal, moisture content and gymnemic acid content were found to be 86.36%, 88.24%, 4.20%, and 26.24% w/w. total phenol and flavonoid content were found to be 0.80% and 1.90%. Microbiological assay showed that E. coli and salmonella were found to be absent whereas total bacterial count and yeast & moulds contents were found to be 650 and 60 cfu/g. quantitative analysis through hptlc revealed the presence of 2.95% w/w of quercetin. Conclusions: In future this study will be helpful for the quantitative analysis of phytoconstituents as well as standardization of the G. sylvestre.

Keywords

• Gymnema sylvestre
• Hptlc
• Phytochemical analysis
• Standardization