In vitro selenium bioaccessibility combined with in vivo bioavailability and bioactivity in Se-enriched microalga (Chlorella sorokiniana) to be used as functional food

Verónica Gómez-Jacinto; Francisco Navarro-Roldán; Inés Garbayo-Nores; Carlos Vílchez-Lobato; Ana Arias Borrego; Tamara García-Barrera

Journal of Functional Foods (Mar 2020)

In vitro selenium bioaccessibility combined with in vivo bioavailability and bioactivity in Se-enriched microalga (Chlorella sorokiniana) to be used as functional food

Verónica Gómez-Jacinto,
Francisco Navarro-Roldán,
Inés Garbayo-Nores,
Carlos Vílchez-Lobato,
Ana Arias Borrego,
Tamara García-Barrera

Affiliations

Verónica Gómez-Jacinto: Environmental and Bioanalytical Chemistry Group, Departament of Chemistry “Profesor José Carlos Vílchez Martín”, Faculty of Experimental Sciences, University of Huelva, Campus El Carmen, 21007 Huelva, Spain; Research Center of Natural Resources, Health and the Environment (RENSMA), University of Huelva, Spain; International Campus of Excellence on Agrofood (ceiA3), University of Huelva, Spain
Francisco Navarro-Roldán: Research Center of Natural Resources, Health and the Environment (RENSMA), University of Huelva, Spain; Department of Integrated Sciences, Cell Biology, Faculty of Experimental Sciences, University of Huelva, Spain
Inés Garbayo-Nores: Research Center of Natural Resources, Health and the Environment (RENSMA), University of Huelva, Spain; Algal Biotechnology Group, CIDERTA, University of Huelva, Parque Huelva Empresarial, 21007 Huelva, Spain
Carlos Vílchez-Lobato: Research Center of Natural Resources, Health and the Environment (RENSMA), University of Huelva, Spain; Algal Biotechnology Group, CIDERTA, University of Huelva, Parque Huelva Empresarial, 21007 Huelva, Spain
Ana Arias Borrego: Environmental and Bioanalytical Chemistry Group, Departament of Chemistry “Profesor José Carlos Vílchez Martín”, Faculty of Experimental Sciences, University of Huelva, Campus El Carmen, 21007 Huelva, Spain; Research Center of Natural Resources, Health and the Environment (RENSMA), University of Huelva, Spain; International Campus of Excellence on Agrofood (ceiA3), University of Huelva, Spain; Corresponding authors at: Department of Chemistry “Professor José Carlos Vílchez Martín”, Faculty of Experimental Sciences, University of Huelva, Campus El Carmen, 21007 Huelva, Spain (T.G. Barrera). Department of Chemistry “Professor José Carlos Vílchez Martín”, Faculty of Experimental Sciences, University of Huelva, Campus El Carmen, 21007 Huelva, Spain (A.A. Borrego).
Tamara García-Barrera: Environmental and Bioanalytical Chemistry Group, Departament of Chemistry “Profesor José Carlos Vílchez Martín”, Faculty of Experimental Sciences, University of Huelva, Campus El Carmen, 21007 Huelva, Spain; Research Center of Natural Resources, Health and the Environment (RENSMA), University of Huelva, Spain; International Campus of Excellence on Agrofood (ceiA3), University of Huelva, Spain; Corresponding authors at: Department of Chemistry “Professor José Carlos Vílchez Martín”, Faculty of Experimental Sciences, University of Huelva, Campus El Carmen, 21007 Huelva, Spain (T.G. Barrera). Department of Chemistry “Professor José Carlos Vílchez Martín”, Faculty of Experimental Sciences, University of Huelva, Campus El Carmen, 21007 Huelva, Spain (A.A. Borrego).

Journal volume & issue: Vol. 66
p. 103817

Abstract

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Speciation of seleno-metabolites and selenoproteins in Se-enriched Chlorella sorokiniana microalgae, in vitro and in vivo extracts has been performed by HPLC-ICP-MS. Selenium bioaccesibility in Se-enriched Chlorela sorokiniana evaluated by in vitro gastrointestinal digestion of the selenized microalga was 81% (79% as SeMet). Mice supplemeted with Se-enriched C. sorokiniana presented increased Se concentration in kidney, reflecting a potencial mechanism of excretion by urine. In addition, selenium bioavailavility, which was in the range of 3–15% depending on the diet was 1.13-fold higher in mice fed with Se-enriched microalgae against the basal diet, but decreased with higher amounts of selenium. Regarding bioactivity (∑Se as selenoproteins in serum/total Se in serum = 76–85% depending on the diet), selenium and selenoproteins (SeP) increased in serum from animals fed high supplemental Se levels. These results showed that Se-enriched algae can be considered as an alternative selenized food for humans, due to their high Se bioavailability.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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