Optimization of the Extraction Process of Curcuminoid by Ionic Liquid-assisted Enzymatic Method

Abstract

In this study, curcuminoid were extracted from turmeric by ionic liquid-assisted enzymatic method. On the basis of single factor test, five factors including enzymatic hydrolysis time, enzymatic hydrolysis temperature, enzymatic hydrolysis pH, enzyme dosage and ionic liquid dosage were selected as response variables, and the extraction rate of curcuminoid was selected as response value. The response surface optimization test was conducted. The optimal process conditions were determined as follows: Enzymatic hydrolysis pH was 5.4, ionic liquid was 20%, enzyme dosage was 21%, enzymatic hydrolysis time was 90 min, enzymatic hydrolysis temperature was 59 ℃. Under these conditions, the theoretical yield of curcuminoid was 5.922%, the actual yield was 5.882%, and the relative error between the theoretical yield and the theoretical yield was 0.04%. This method can effectively extract curcuminoid from turmeric, providing reference for the development and utilization of curcumin resources.

Keywords

• curcuminoid
• ionic liquid
• cellulase
• extraction technique