Calebin-A induces cell cycle arrest in human colon cancer cells and xenografts in nude mice

Wen-Shiung Liou; Chen Lin; Pei-Sheng Lee; Nagabhushanam Kalyanam; Chi-Tang Ho; Min-Hsiung Pan

Journal of Functional Foods (Oct 2016)

Calebin-A induces cell cycle arrest in human colon cancer cells and xenografts in nude mice

Wen-Shiung Liou,
Chen Lin,
Pei-Sheng Lee,
Nagabhushanam Kalyanam,
Chi-Tang Ho,
Min-Hsiung Pan

Affiliations

Wen-Shiung Liou: Department of Obstetrics and Gynecology, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan 81362; Department of Obstetrics and Gynecology, National Defense Medical Center, Taipei, Taiwan 114; National Yang-Ming University, Taipei, Taiwan 11221
Chen Lin: Institute of Food Science and Technology, National Taiwan University, Taipei, Taiwan 10617
Pei-Sheng Lee: Institute of Food Science and Technology, National Taiwan University, Taipei, Taiwan 10617
Nagabhushanam Kalyanam: Sabinsa Corporation, 20 Lake Drive, East Windsor, NJ 08520, USA
Chi-Tang Ho: Department of Food Science, Rutgers University, New Brunswick, NJ 08901, USA
Min-Hsiung Pan: Institute of Food Science and Technology, National Taiwan University, Taipei, Taiwan 10617; Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, Taiwan 40402; Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan 41354; Corresponding author. Institute of Food Science and Technology, National Taiwan University, No.1, Section 4, Roosevelt Road, Taipei 10617, Taiwan. Fax: (886) 2 33661771.

Journal volume & issue: Vol. 26
pp. 781 – 791

Abstract

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Calebin-A is a curcuminoid compound isolated from turmeric. Herein, we investigated the antiproliferative effect of calebin-A in human colon cancer cells and demonstrated that calebin-A had a more potent inhibition on cell growth compared with curcumin and tetrahydrocurcumin. In this study, calebin-A caused G2/M cycle arrest through decreasing the protein levels of cdc25A, cyclin B, cyclin A and cdc2 and increasing cyclin-dependent kinases inhibitors (CDKI) such as p53 and p21. Moreover, calebin-A increased reactive oxygen species (ROS) levels, triggered a DNA damage response and up-regulated the phosphorylation of H2AX, chk1, chk2. Furthermore, intraperitoneally administrated calebin-A on HCT116 tumour xenografts in vivo significantly decreased tumour volumes as well as reduced tumour sizes and proliferating cell nuclear antigen (PCNA) protein levels. These results have an important implication for using calebin-A as a functional food ingredient for cancer prevention.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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