Preparation of chitosan matrices with ferulic acid: physicochemical characterization and relationship on the growth of Aspergillus parasiticus

Abstract

Antioxidant, anticarcinogenic, and antimicrobial properties have been reported for ferulic acid (FA), therefore, its application interests both food and agriculture research. FA was immobilized in different chitosan (CS) matrices, physicochemicaly characterized and the effect on Aspergillus parasiticus ecological parameters evaluated. Nanoparticles (Nparticles), microparticles (Mparticles) and microcapsules (Mcapsules) of 35–40 nm, 30–40 μm, and 20 μm, respectively were obtained; FA incorporation in matrices affected their morphology, physicochemical properties, and their fungistatic effect. The effect of the particles was dependent on the matrix exposed. Nparticles and Mparticles showed high FA immobilization efficiency as well as a good fungistatic effect against A. parasiticus: Radial growth at 168 h was 28.46 ± 1.01 and 28.84 ± 1.36 and the inhibition of spore germination at 30 h was 57.44 ± 0.22 and 55.74 ± 2.19, for Nparticles and Mparticles, respectively compared with control cultures. Abnormalities in mycelium, hyphae, and spores morphology were observed, as well as low sporulation due particle interaction with the fungus.

Keywords

• Ferulic acid
• chitosan
• Aspergillus parasiticus
• controlled release matrices