Fermentation (Dec 2022)

Kinetic Modeling of an Enzyme Membrane Reactor for the Selective Production of Oligosaccharides

  • Shusaku Asano,
  • Yosuke Muranaka,
  • Taisuke Maki,
  • Koki Ikeda,
  • Kazuhiro Mae

DOI
https://doi.org/10.3390/fermentation8120701
Journal volume & issue
Vol. 8, no. 12
p. 701

Abstract

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An enzyme membrane reactor is an attractive tool for producing oligosaccharides from biomass-based polysaccharides. However, kinetic modeling and reactor design based on the rate equations have rarely been reported for enzyme membrane reactors because of the difficulty in tracing the depolymerization process. In this study, a simplified reaction model based on Michaelis–Menten-type kinetics has been built to simulate the enzyme membrane reactor. Ramping various species into reactant, target, and byproduct worked well for discussing reactor performance. The use of a membrane with a molecular weight cut-off (MWCO) of 10 kDa with continuous feeding of the reactant was suggested for the efficient production of chitosan hexamer and pentamer by enzymatic hydrolysis of chitosan.

Keywords