Inhibitory effect of ultrasonicated sesame meal on fat accumulation of 3T3-L1 adipocytes and oleic acid induced HepG2 hepatocytes

Abstract

Antioxidant activities of an aqueous extract of sesame meal (SM-HW) and an ultrasonicated sesame meal (SSM-HW) were evaluated and anti-obesity activity of SSM-HW was determined using in vitro assays. In addition, metabolite analyses were conducted using GC/MS to determine major non-volatile compounds in SM-HW and SSM-HW. Antioxidant activities of SSM-HW were significantly higher than those of SM-HW. SSM-HW had higher concentrations of some organic acids and amino acids than SM-HW. Differentiation of 3T3-L1 adipocytes was decreased by 20 % after treatment with 10 μg/mL SSM-HW compared to control. Expression levels of several adipocyte marker genes and proteins were significantly decreased compared to control (p < 0.05) when 3T3-L1 adipocytes were treated with SSM-HW. In addition, SSM-HW decreased fat accumulation in HepG2 hepatocytes and expression of adipocyte marker genes and proteins. Overall, SSM-HW had improved antioxidant power and effectively inhibited fat accumulation in 3T3-L1 adipocytes and oleic acid-induced HepG2 hepatocytes.

Keywords

• Ultrasonicated sesame meal
• Antioxidant activity
• 3T3-L1 adipocytes
• HepG2 hepatocyte
• Non-alcoholic fatty liver disease