Cluster of differentiation 14 and toll-like receptor 4 are involved in the anti-inflammatory effects of tyrosol

Chao-Yuan Chang; I-Tao Huang; Hung-Jen Shih; Ya-Ying Chang; Ming-Chang Kao; Ping-Chen Shih; Chun-Jen Huang

Journal of Functional Foods (Feb 2019)

Cluster of differentiation 14 and toll-like receptor 4 are involved in the anti-inflammatory effects of tyrosol

Chao-Yuan Chang,
I-Tao Huang,
Hung-Jen Shih,
Ya-Ying Chang,
Ming-Chang Kao,
Ping-Chen Shih,
Chun-Jen Huang

Affiliations

Chao-Yuan Chang: Department of Anesthesiology, Wan Fang Hospital, Taipei Medical University, 111, Sec. 3, Xinglong Rd., Wenshan Dist., Taipei 116, Taiwan; Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, 250, Wuxing St., Taipei 110, Taiwan
I-Tao Huang: Medical Education Unit, Rockhampton Hospital, Central Queensland Hospital and Health Service, Queensland Government, Canning St., Rockhampton, QLD 4700, Australia; School of Public Health, Faculty of Medicine, University of Queensland, Herston Campus, 288, Herston Rd., Herston, QLD 4006, Australia
Hung-Jen Shih: Department of Urology, Wan Fang Hospital, Taipei Medical University, 111, Sec. 3, Xinglong Rd., Wenshan Dist., Taipei 116, Taiwan; School of Medicine, College of Medicine, Taipei Medical University, 250, Wuxing St., Taipei 110, Taiwan
Ya-Ying Chang: Department of Anesthesiology, Far Eastern Memorial Hospital, 21, Sec. 2, Nanya S. Rd., Banciao Dist., New Taipei City 220, Taiwan
Ming-Chang Kao: Department of Anesthesiology, Taipei Tzu Chi Hospital, 289, Jianguo Rd., Xindian Dist., New Taipei City 231, Taiwan
Ping-Chen Shih: Department of Anesthesiology, Taipei Tzu Chi Hospital, 289, Jianguo Rd., Xindian Dist., New Taipei City 231, Taiwan
Chun-Jen Huang: Department of Anesthesiology, Wan Fang Hospital, Taipei Medical University, 111, Sec. 3, Xinglong Rd., Wenshan Dist., Taipei 116, Taiwan; Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, 250, Wuxing St., Taipei 110, Taiwan; Corresponding author at: Department of Anesthesiology, Wan Fang Hospital, 111, Sec. 3, Xinglong Rd., Wenshan Dist., Taipei 116, Taiwan.

Journal volume & issue: Vol. 53
pp. 93 – 104

Abstract

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We investigated effects of tyrosol on upstream pathway regulating lipopolysaccharide (LPS)-induced inflammatory response, including cluster of differentiation 14 (CD14)-mediated LPS–macrophage binding, toll-like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD2) expression and subsequent myeloid differentiation primary response gene 88 (MyD88) and TIR-domain-containing adapter-inducing interferon-β (TRIF) recruitments. Flow cytometry revealed that, compared with LPS-treated RAW264.7 cells (LPS group), those treated with LPS and tyrosol (1.2 mM) [LPS+T(1.2) group] demonstrated lower LPS–macrophage binding and membrane-bound CD14 (mCD14) and TLR4/MD2 expression (all p < 0.05). Immunoprecipitation/immunoblotting assay revealed lower MyD88 and TRIF concentrations in the LPS+T(1.2) group than in the LPS group (both p < 0.05). Soluble CD14 concentration was higher in the LPS+T(1.2) group than in the LPS group (p = 0.013); protease inhibition counteracted this effect. Thus, tyrosol inhibits LPS–macrophage binding, mCD14 and TLR4/MD2 expression, and MyD88 and TRIF recruitment, all possibly by enhancing protease-mediated mCD14 cleavage.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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