Protective effects of phenolic constituents from Cytisus multiflorus, Lamium album L. and Thymus citriodorus on liver cells

Olívia R. Pereira; Rocio I.R. Macias; María J. Perez; Jose J.G. Marin; Susana M. Cardoso

Journal of Functional Foods (Jul 2013)

Protective effects of phenolic constituents from Cytisus multiflorus, Lamium album L. and Thymus citriodorus on liver cells

Olívia R. Pereira,
Rocio I.R. Macias,
María J. Perez,
Jose J.G. Marin,
Susana M. Cardoso

Affiliations

Olívia R. Pereira: Departamento de Tecnologias de Diagnóstico e Terapêutica, Escola Superior de Saúde, Instituto Politécnico de Bragança, Av. D. Afonso V, 5300-121 Bragança, Portugal; CIMO, Escola Superior Agrária, Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5301-855 Bragança, Portugal
Rocio I.R. Macias: Laboratory of Experimental Hepatology and Drug Targeting (HEVEFARM), Biomedical Research Institute of Salamanca (IBSAL), CIBERehd, University of Salamanca, Spain
María J. Perez: Laboratory of Experimental Hepatology and Drug Targeting (HEVEFARM), Biomedical Research Institute of Salamanca (IBSAL), CIBERehd, University of Salamanca, Spain; Hospital Universitario de Salamanca, Instituto de Estudios de Ciencias de la Salud de Castilla y León, Salamanca, Spain
Jose J.G. Marin: Laboratory of Experimental Hepatology and Drug Targeting (HEVEFARM), Biomedical Research Institute of Salamanca (IBSAL), CIBERehd, University of Salamanca, Spain; Corresponding authors. Addresses: Department of Physiology and Pharmacology, IBSAL, Campus Miguel de Unamuno EID S-09, 37007 Salamanca, Spain. Fax: +34 923 294669 (J.J.G. Marin). CERNAS, Escola Superior Agrária, Instituto Politécnico de Coimbra, Bencanta, 3040-316 Coimbra, Portugal. Tel.: +351 239 802940; fax: +351 273 239 802979 (S.M. Cardoso).
Susana M. Cardoso: CIMO, Escola Superior Agrária, Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5301-855 Bragança, Portugal; CERNAS, Escola Superior Agrária, Instituto Politécnico de Coimbra, Bencanta, 3040-316 Coimbra, Portugal; Corresponding authors. Addresses: Department of Physiology and Pharmacology, IBSAL, Campus Miguel de Unamuno EID S-09, 37007 Salamanca, Spain. Fax: +34 923 294669 (J.J.G. Marin). CERNAS, Escola Superior Agrária, Instituto Politécnico de Coimbra, Bencanta, 3040-316 Coimbra, Portugal. Tel.: +351 239 802940; fax: +351 273 239 802979 (S.M. Cardoso).

Journal volume & issue: Vol. 5, no. 3
pp. 1170 – 1179

Abstract

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The present study investigated the antioxidant and cytoprotective effects of purified ethanolic extracts of Cytisus multiflorus, Lamium album L. and Thymus citriodorus plants. These extracts showed high antioxidant activity in DPPH and reducing power assays. Using a model of chemical stress induced by potassium dichromate (DK) in human hepatoblastoma HepG2 cells, 50 μg/mL of C. multiflorus, L. album and T. citriodorus extracts decreased the rate of reactive oxygen species (ROS) production by 35%, 26% and 20%, respectively, when exposed to 25 μM of DK. This effect was also observed for the treatment of cells with individual polyphenolic compounds determined in the extracts, or with mixtures prepared with individual polyphenolic compounds simulating the phenolic composition of the extracts. Additionally, the purified ethanolic extracts and the prepared polyphenolic mixtures showed a cytoprotective effect against DK-induced toxicity. The overall results emphasize the contribution of polyphenols in antioxidant and cytoprotective properties of the studied plants.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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