Ameliorating mechanism of nuciferine on high-fat diet-induced dyslipidemia and hepatic steatosis by regulating intestinal absorption and serum extracellular vesicles in rats

Xiaochen Dong; Shengnan Zhu; Jun Liu; Zonghua Dong; Feng Guan; Aichun Xu; Jin Zhao; Jian Ge

Journal of Functional Foods (Aug 2022)

Ameliorating mechanism of nuciferine on high-fat diet-induced dyslipidemia and hepatic steatosis by regulating intestinal absorption and serum extracellular vesicles in rats

Xiaochen Dong,
Shengnan Zhu,
Jun Liu,
Zonghua Dong,
Feng Guan,
Aichun Xu,
Jin Zhao,
Jian Ge

Affiliations

Xiaochen Dong: College of Life Sciences, China Jiliang University, 258 XueYuan Street, XiaSha Higher Education Zone. Hangzhou, 310018 Zhejiang Province, People’s Republic of China
Shengnan Zhu: College of Life Sciences, China Jiliang University, 258 XueYuan Street, XiaSha Higher Education Zone. Hangzhou, 310018 Zhejiang Province, People’s Republic of China
Jun Liu: College of Life Sciences, China Jiliang University, 258 XueYuan Street, XiaSha Higher Education Zone. Hangzhou, 310018 Zhejiang Province, People’s Republic of China
Zonghua Dong: College of Life Sciences, China Jiliang University, 258 XueYuan Street, XiaSha Higher Education Zone. Hangzhou, 310018 Zhejiang Province, People’s Republic of China
Feng Guan: College of Life Sciences, China Jiliang University, 258 XueYuan Street, XiaSha Higher Education Zone. Hangzhou, 310018 Zhejiang Province, People’s Republic of China
Aichun Xu: College of Life Sciences, China Jiliang University, 258 XueYuan Street, XiaSha Higher Education Zone. Hangzhou, 310018 Zhejiang Province, People’s Republic of China
Jin Zhao: College of Life Sciences, China Jiliang University, 258 XueYuan Street, XiaSha Higher Education Zone. Hangzhou, 310018 Zhejiang Province, People’s Republic of China
Jian Ge: Corresponding author.; College of Life Sciences, China Jiliang University, 258 XueYuan Street, XiaSha Higher Education Zone. Hangzhou, 310018 Zhejiang Province, People’s Republic of China

Journal volume & issue: Vol. 95
p. 105182

Abstract

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We investigated the effects of nuciferine rich in Lotus leaf on the amelioration of hepatic steatosis and dyslipidemia of hyperlipidemic rats, and explored the underlying mechanism on miRNAs and genes expression. Serum extracellular vesicles (EVs) from rats were prepared and characterized, and interactions between miRNAs and target mRNAs were validated in 293T cell line with the double luciferase reporter gene assay. Results showed that nuciferine significantly induced the expression of PPARα, ABCA1, CYP7A1, LXRα in liver and ABCG5 in intestine, while NPC1L1 expression was markedly reduced. In vitro, double luciferase reporter assays showed that miRNA-764-5p, miRNA-758-3p, and miRNA-126-5p interacted with the target mRNAs of PPARα, ABCA1, and CYP7A1, respectively. And the effect of nuciferine on lipid metabolism in rats was related to regulate intestinal absorption and serum EVs miRNAs. These miRNAs were able to reduce lipid levels by targeting lipid metabolism genes and up-regulating PPARα-LXRα-ABCA1 pathway and CYP7A1 expression.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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