Journal of Functional Foods (Jan 2020)

Lipid-rich extract from Mexican avocado (Persea americana var. drymifolia) induces apoptosis and modulates the inflammatory response in Caco-2 human colon cancer cells

  • Mónica Lara-Márquez,
  • Marisol Báez-Magaña,
  • Cristina Raymundo-Ramos,
  • Paul A. Spagnuolo,
  • Lourdes Macías-Rodríguez,
  • Rafael Salgado-Garciglia,
  • Alejandra Ochoa-Zarzosa,
  • Joel E. López-Meza

Journal volume & issue
Vol. 64
p. 103658

Abstract

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Colorectal cancer (CRC) is the third most common type of cancer worldwide. Chemotherapy used in CRC patients has severe side effects. As an alternative, the search to identify natural compounds increases the opportunity to select therapeutics. In this work, the cytotoxic effect of a lipid extract (LEAS) from Mexican native avocado seed (Persea americana var. drymifolia) on colon cancer cells Caco-2 was determined. LEAS was cytotoxic towards Caco-2 cells (IC50 = 28 µg/ml), inducing apoptosis through the activation of caspases 8 and 9. Also, LEAS induced loss of membrane mitochondrial potential, inhibited fatty acid oxidation and increased the superoxide production and mitochondrial ROS. Furthermore, LEAS stimulated secretion of cytokines IL-6 (~500%), IL-8 (~400%) and IL-10 (~150%); whereas IL-1β secretion was inhibited (~50%). The results suggest that LEAS induces apoptosis on Caco-2 cells, indicating that avocado is a source of functional food products that can reduce the risk for development of cancer.

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