Protective effect and mechanism of Muntingia calabura Linn. fruit ethanolic extract against vascular endothelial growth factor production in nickel-stimulated hepatocellular carcinoma cells

Jau-Tien Lin; Yuan-Yen Chang; Yi-Chen Chen; Li-Chun Kuo; Deng-Jye Yang

Journal of Functional Foods (Aug 2018)

Protective effect and mechanism of Muntingia calabura Linn. fruit ethanolic extract against vascular endothelial growth factor production in nickel-stimulated hepatocellular carcinoma cells

Jau-Tien Lin,
Yuan-Yen Chang,
Yi-Chen Chen,
Li-Chun Kuo,
Deng-Jye Yang

Affiliations

Jau-Tien Lin: Department of Medical Applied Chemistry, Chung Shan Medical University, and Department of Medical Education, Chung Shan Medical University Hospital, 110, Section 1, Jianguo N. Road, Taichung 40201, Taiwan
Yuan-Yen Chang: Department of Microbiology and Immunology, School of Medicine, Chung-Shan Medical University, and Clinical Laboratory, Chung Shan Medical University Hospital, 110, Section 1, Jianguo N. Road, Taichung 40201, Taiwan
Yi-Chen Chen: Department of Animal Science and Technology, National Taiwan University, 1, Section 4, Roosevelt Road, Taipei 106, Taiwan
Li-Chun Kuo: Department of Health Diet and Industry Management, Chung Shan Medical University, 110, Section 1, Jianguo N. Road, Taichung 40201, Taiwan
Deng-Jye Yang: Institute of Food Safety and Health Risk Assessment, National Yang-Ming University, 155, Sec.2, Linong Street, Taipei 11221, Taiwan; Department of Nutrition and Master Program for Food and Drug Safety, China Medical University, 91, Hsueh-Shih Road, Taichung 40402, Taiwan; Corresponding author at: Institute of Food Safety and Health Risk Assessment, National Yang-Ming University, 155, Sec.2, Linong Street, Taipei 112, Taiwan.

Journal volume & issue: Vol. 47
pp. 343 – 349

Abstract

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The protective effect of the M. calabura Linn. fruit ethanolic extract (MFEE) against nickel (Ni)-induced vascular endothelial growth factor (VEGF) expression in hepatocellular carcinoma (HepG2) cells was investigated. MFEE contained 4 flavonoids: epicatechin, rutin, diosmin and luteolin, and 11 phenolic acids: gallic acid, gentisic acid, p-hydroxybenzoic acid, vanillic acid, p-coumaric acid, ferulic acid, sinapic acid, syringic acid, p-anisic acid and rosmarinic acid. Substantial VEGF expression was assayed in Ni-stimulated HepG2 cells, which was significantly suppressed through MFEE treatment via down-regulating Raf-1 proto-oncogene, serine/threonine kinase (RAF1) /mitogen-activated protein kinase 1/2 (MEK1/2)/extracellular-signal-regulated kinase 1/2 (ERK1/2), and Janus kinase 2 (JAK2)/signal transducers and activators of transcription 3 (STAT3) pathways as well as inhibiting hypoxia-inducible factor (HIF)-1α expression down-regulated by phosphatidylinositol-3-kinase (PI3K)/ protein kinase B (AKT)/mammalian target of rapamycin (mTOR) pathway. The results showed that MFEE should have the potential to lower liver cancer risk in the Ni-polluted environment by suppressing VEGF expression.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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