Paralytic shellfish poisoning toxin detection based on cell-based sensor and non-linear signal processing model

Abstract

Paralytic shellfish poisoning (PSP) toxin is widely contained in seafood and its product. It brings great harm to human health when people eat it. During the past two decades, shellfish toxin quantitative analysis methods develop fast, such as mouse bioassay (MBA), chemical analysis method, immunoassay and cell-based assay (CBA). However, there are some disadvantages in these analytical methods such as time consuming, high cost. Therefore, a more suitable method is in demand. In this study, a novel method using high-frequency surface acoustic wave resonator (SAWR)-based sensor combined with living cells was developed for shellfish toxin continuous monitoring. The mouse tongue isolated taste bud (MTITB) cells cell lines were used as the sensing elements to establish the sensor device for PSP toxin carbamoyl toxin (CT) quantitative monitoring. The sensor device is in serial with SAWR. SAWR output frequency was recorded for detecting characterization. HEK293 cell lines were cultured on electrode and utilized as negative control. The results showed that MTITB cell-based sensor had selective responses to CT. This method is promising in PSP toxins rapid quantitative determination.

Keywords

• cell-based sensor
• paralytic shellfish poisoning toxin
• carbamoyl toxin
• sawr
• frequency