High-Gossypol Whole Cottonseed Exhibited Mediocre Rumen Degradability and Less Microbial Fermentation Efficiency than Cottonseed Hull and Cottonseed Meal with an In Vitro Gas Production Technique

Abstract

To explore whether or not the gossypol varied in cottonseed by-products affect rumen degradability and fermentation efficiency, an in vitro cumulative gas production experiment was applied with mixed rumen microorganism to compare rumen fermentation characteristics of whole cottonseed (WCS, n = 3 samples), cottonseed meal (CSM, n = 3 samples), and cottonseed hull (CSH, n = 2 samples). The five-replicate fermentation per sample per incubation time continuously lasted for 0.5, 1.5, 3, 6, 12, 24, 36, and 48 h with an automated gas production recording system. Regardless of distinct nutrient differences, the free gossypol level in these cottonseed by-products ranked: WCS > CSH > CSM. After 48 h of incubation, the in vitro dry matter degradability and ammonia N concentration ranked as: CSM > WCS > CSH. The cumulative gas production and total volatile fatty acid (VFA) levels in the culture fluids ranked: CSM > CSH > WCS, in which the average production rate ranked: CSM > WCS > CSH. Regarding the molar VFA pattern, WCS in comparison with CSH and CSM presented the lowest production of non-glucogenic acids (e.g., acetate) and exhibited the highest fermentation efficiency of energy from carbohydrates to VFAs. There was a significant negative correlation between the gossypol content and cumulative gas and total VFA production, suggesting that the greater gossypol in cottonseed by-products, the more detrimental effect occurred for rumen fermentation. In a brief, WCS exhibited mediocre rumen degradability and less microbial fermentation efficiency than CSH and CSM, depending on their gossypol levels.

Keywords

• cottonseed by-products
• gossypol
• in vitro incubation
• gas production kinetics
• ruminal fermentation characteristics