Anti-helminthic Activity of Consolida orientalis (Gay) Schröd. on Caenorhabditis elegans Nematodes and Determination of Possible Active Ingredients

Abstract

Helminthiasis is an important parasitic disease, many of which are zoonotic, particularly common in developing countries and, in countries with hot and humid climates. Intestinal parasites can cause significant manifestations at all levels of the gastrointestinal tract, as well as anemia and growth retardation. This study aims to demonstrate the anti-helminthic effect of Consolida orientalis on Caenorhabditis elegans, which is a helminth model, and introduce new chemotherapeutic candidate substances with anti-helminthic effect to the literature by identifying possible active ingredients with GC-MS analysis. In our study, flower, leaf, stem and aerial part plant extracts of Consolida orientalis were used at 40 mg/mL, 20 mg/mL, 10 mg/mL, 5 mg/mL, 2.5 mg/mL, 1.25 mg/mL concentrations. In addition, possible active ingredients found in plant extracts were determined by GC-MS analysis. As a result of the study, it was determined that the aerial part, leaf and stem extract of the first four concentrates (40 mg/mL, 20 mg/mL, 10 mg/mL, 5 mg/mL) of Consolida orientalis were more effective than pyrantel pamoate at a concentration of 5 mg/mL. According to our study findings, chemotherapeutics such as Dihidrocarvone and 2(3H)-Benzoxazolone with new antihelmintic-antiparasitic activity are thought to contribute to further research.

Keywords

• anti-helminthic activity
• caenorhabditis elegans
• consolida orientalis
• dihidrocarvone
• 2(3h)-benzoxazolone