Preventive Effect of Lactobacillus paracasei FZU103 on Alcoholic Liver Injury in Mice

LIANG Zihua, LI Jiayi, XIE Linhui, YANG Ziyi, YOU Shize, WU Chao, LI Wenlong, AI Lianzhong, NI Li, LÜ Xucong, CHEN Youting

doi:10.7506/spkx1002-6630-20230729-329

Shipin Kexue (Apr 2024)

Preventive Effect of Lactobacillus paracasei FZU103 on Alcoholic Liver Injury in Mice

LIANG Zihua, LI Jiayi, XIE Linhui, YANG Ziyi, YOU Shize, WU Chao, LI Wenlong, AI Lianzhong, NI Li, LÜ Xucong, CHEN Youting

Affiliations

LIANG Zihua, LI Jiayi, XIE Linhui, YANG Ziyi, YOU Shize, WU Chao, LI Wenlong, AI Lianzhong, NI Li, LÜ Xucong, CHEN Youting: (1. Institute of Food Science and Technology, College of Biological Science and Technology, Fuzhou University, Fuzhou 350108, China; 2. Food Nutrition and Health Research Center, School of Advanced Manufacturing, Fuzhou University, Jinjiang 362200, China; 3. The First Clinical Medical College, Fujian Medical University, Fuzhou 350004, China; 4. Shanghai Engineering Research Center of Food Microbiology, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China; 5. Department of Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital, Fujian Medical University, Fuzhou 350004, China)

DOI: https://doi.org/10.7506/spkx1002-6630-20230729-329
Journal volume & issue: Vol. 45, no. 7
pp. 135 – 144

Abstract

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Objective: To explore the preventive effect of Lactobacillus paracasei FZU103 (LP-FZU103) on alcoholic liver injury (ALI). Methods: Altogether 36 specific pathogen free- (SPF-) grade ICR mice were randomly divided into three groups: control, model and experimental (LP-FZU103 intervention). After the six-week experiment, body mass, organ coefficients, serum and liver biochemical indexes, liver histopathology and inflammatory cytokines, the transcription of liver function-related genes and intestinal flora composition were measured. Results: Compared with the model group, intervention of LP-FZU103 improved the organ coefficients and pathological liver damage in ALI mice, significantly reduced the levels of total cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C) as well as the activity of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the serum, increased the serum concentration of high-density lipoprotein cholesterol (HDL-C), significantly increased the activity of catalase (CAT) and superoxide dismutase (SOD) and the content of glutathione (GSH) in the liver, and decreased MDA content and interleukin-6 (IL-6), interferon-γ (IFN-γ) levels in the liver. Moreover, LP-FZU103 intervention significantly up-regulated the mRNA expression level of the lipid metabolism-related gene Ldlr and down-regulated the mRNA expression level of Acc1, Hmgcr and Cd36 as well as increased the relative abundance of beneficial bacteria such as Lactobacillus johnsonii, Lactobacillus reuteri and Lactobacillus paracasei in the gut of mice. Conclusion: LP-FZU103 intervention can prevent and control the occurrence of alcoholic liver injury in mice, which is closely related to the improved intestinal flora and liver metabolic function.

lactobacillus paracasei; alcoholic liver injury; oxidative stress; liver function-related genes; intestinal flora

Published in Shipin Kexue

ISSN: 1002-6630 (Print)
Publisher: China Food Publishing Company
Country of publisher: China
LCC subjects: Technology: Chemical technology: Food processing and manufacture
Website: https://www.spkx.net.cn/EN/1002-6630/home.shtml

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