Foods and Raw Materials (Sep 2014)

THEORY AND PRACTICE OF PRION PROTEIN ANALYSIS IN FOOD PRODUCTS

  • Prosyekov A.

DOI
https://doi.org/10.12737/5467
Journal volume & issue
Vol. 2, no. 2
pp. 106 – 120

Abstract

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The article presents the results of the research on methods of identification and quantitative determination of prion proteins in biological samples and multicomponent mixtures based on them. Analysis of nucleotide sequence of DNA encoding the PRNP gene of the prion protein, including phylogenetic and comparative analysis of nucleotide sequences of normal and pathogenic prion protein in cattle, was performed. Oligonucleotide primers for amplification of the PRNP gene of pathogenic prion protein were designed and synthesized. The high specificity of the developed test system was confirmed.

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