Chemical profiling and in-vitro α-amylase inhibitory activity of Sesbania sesban and Sesbania grandiflora seeds

Abstract

ABSTRACTS. sesban is mainly used as economical purpose and S. grandiflora is known for medicinal as well as economical. Chemical characterization and antidiabetic activity of seed extracts were not explored yet. Seeds of S. sesban and S. grandiflora were separated in seed coat, germ, and endosperm by domestic grinder mixer. Successive soxhlet extraction of separated parts of Sesbania seed were carried out. Chemical profiling of methanolic extract was carried out by GC-MS, HPLC, and in vitro alpha amylase inhibitory assay. D-pinitol was found to be maximum (48.39%) in seed coat and minimum in seed (8.35%) of methanolic extracts of Sesbania seed. Alpha-amylase inhibitory activity of methanolic extracts of different parts were also studied in which DSSh, DSSp, & DSSe showed similar IC50 to acarbose, antidiabetic drugs. The result showed that S. sesban and S. grandiflora seed and endosperm is rich in health promoting mineral like Na (241 mg/100 gm and 238 mg/100 gm), K (103.1 mg/100 gm) and (60.2 mg/100 gm), Ca (525 mg/100 gm) and (500 mg/100 gm) and Fe (67.5 and 91.9 mg/100 gm). Hence, it is similar to some edible legumes which possess bioactive compound with therapeutic value and can be efficiently explored for the development of new functional or nutraceutically valued food products.

Keywords

• Sesbania
• Alpha-amylase activity
• nutritional
• D-Pinitol
• IC50
• Acarbose