FACTORS AFFECTING ANTIBIOFILM PROPERTIES OF MICROBIAL SURFACTANTS
TETIANA P. PIROG ,
LILIYA V. KLUCHKA ,
DARIA A. LYTSAI,
VIKTOR P. STABNIKOV
Affiliations
TETIANA P. PIROG
1. National University of Food Technologies, Department of Biotechnology and Microbiology, 68, Volodymyrskaya Str. Kyiv, 01601, Ukraine 2. Institute of Microbiology and Virology NAS of Ukraine,154, Zabolotny Str., Kyiv, 03143, Ukraine
LILIYA V. KLUCHKA
1. National University of Food Technologies, Department of Biotechnology and Microbiology, 68, Volodymyrskaya Str. Kyiv, 01601, Ukraine
DARIA A. LYTSAI
1. National University of Food Technologies, Department of Biotechnology and Microbiology, 68, Volodymyrskaya Str. Kyiv, 01601, Ukraine
VIKTOR P. STABNIKOV
1. National University of Food Technologies, Department of Biotechnology and Microbiology, 68, Volodymyrskaya Str. Kyiv, 01601, Ukraine
Antibiofilm activity of surface-active substances (SAS) synthesized by Rhodococcus erythropolis, Acinetobacter calcoaceticus, or Nocardia vaccinia was studied. The strains were cultivated using carbon ethanol, glycerol, hexadecane, sunflower oil, and carbon-containing wastes from biodiesel production as carbon sources. Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, and yeasts Candida albicans and Candida utilis were used as testing cultures. The antibiofilm activity of biosurfactants depended on the source and concentration of carbon source and duration of cultivation. The addition of calcium cations to media significantly increased antibiofilm activity. Replacement of pure sources of carbon for wastes provided the opportunity to receive biosurfactants with high antibiofilm properties on inexpensive substrates. Destruction of biofilms was the same in cases when supernatant or purified SAS was used.
