In vitro antioxidant potential and inhibitory effect of hydro-ethanolic extract from African black velvet tamarind (Dialium indium) pulp on type 2 diabetes linked enzymes
Olakunle Bamikole Afolabi,
Omotade Ibidun Oloyede,
Abiodun Ayodele Ojo,
Amos Adeyinka Onansanya,
Shadrach Oludare Agunbiade,
Bashir Olaitan Ajiboye,
Jonathan Johnson,
Omolara Abosede Peters
Affiliations
Olakunle Bamikole Afolabi
Phytomedicine and Toxicology Laboratory, Biochemistry Unit, Department of Chemical Science, College of Science, Afe-Babalola University. P.M.B 5454, Ado- Ekiti, Ekiti State
Omotade Ibidun Oloyede
Department of Biochemistry, Ekiti State University, P.M.B 5363, Ado‑Ekiti
Abiodun Ayodele Ojo
Department of Chemical Science, College of Science, Afe-Babalola University. P.M.B 5454, Ado- Ekiti, Ekiti State
Amos Adeyinka Onansanya
Molecular and Biotechnology Laboratory, Biochemistry Unit, Department of Chemical Science, College of Science, Afe-Babalola University. P.M.B 5454, Ado- Ekiti, Ekiti State
Shadrach Oludare Agunbiade
Nutrition and Food Science Laboratory, Biochemistry Unit, Department of Chemical Science, College of Science, Afe-Babalola University. P.M.B 5454, Ado- Ekiti, Ekiti State
Bashir Olaitan Ajiboye
Phytomedicine and Toxicology Laboratory, Biochemistry Unit, Department of Chemical Science, College of Science, Afe-Babalola University. P.M.B 5454, Ado- Ekiti, Ekiti State
Jonathan Johnson
Department of Chemical Science, College of Science, Afe-Babalola University. P.M.B 5454, Ado- Ekiti, Ekiti State
Omolara Abosede Peters
Department of Chemical Science, College of Science, Afe-Babalola University. P.M.B 5454, Ado- Ekiti, Ekiti State
The alarming rate of diabetes mellitus (DM) globally is bothersome and has drawn the search light of researchers on naturally endowed phytonutrients being an alternative in managing the menace. Therefore, the current study was designed to investigate some antioxidant parameters embedded in the extract of Dialium indium (DI) fruit pulp and also, to elucidate its antidiabetic potentials through the inhibition of two key carbohydrate-metabolizing enzymes such as α-amylase and intestinal α-glucosidase. Hydro-ethanolic extract of DI fruit pulp was used for the antioxidants and enzyme inhibitory bioassays through various convectional antioxidant assay methods in vitro. In the results, total phenolic content of the extract had; 6.74 ±3.38 mg GAE.g-1, total flavonoid contents; 0.02 ±0.01 mg QE.g-1 and FRAP; 0.84 ±0.47 mg AAE.g-1 dried sample. Also, there was a marked significant (p <0.05) difference observed in the inhibition of α-amylase and intestinal α-glucosidase by the different concentrations of the extract used in concentration-dependent manner with their different EC50. The inhibition demonstrated against these two carbohydrate metabolizing enzymes possibly could be through the embedded antioxidant potentials of the fruit pulp and this if properly harnessed, it could be helpful in the management of type 2 diabetes.
