Peptides isolated from black soybean synergistically inhibit the activity of angiotensin converting enzyme (ACE)

Ben Shao; Xinyu Huang; Mengdan Xu; Dawei Cheng; Xueling Li; Meiqing Li

Journal of Functional Foods (Jul 2023)

Peptides isolated from black soybean synergistically inhibit the activity of angiotensin converting enzyme (ACE)

Ben Shao,
Xinyu Huang,
Mengdan Xu,
Dawei Cheng,
Xueling Li,
Meiqing Li

Affiliations

Ben Shao: Anhui Engineering Laboratory for Agro-products Processing, Anhui Agricultural University, Hefei 230036, China; Anhui Engineering Research Center of Functional Food for Plant Active Peptides, Hefei 230036, Anhui, China; College of Tea and Food Science & Technology, Anhui Agricultural University, Hefei 230036, Anhui, China; Key Laboratory of Jianghuai Agricultural Product Fine Processing and Resource Utilization, Ministry of Agriculture and Rural Affairs, Anhui Agricultural University, Hefei 230036, China
Xinyu Huang: Anhui Engineering Laboratory for Agro-products Processing, Anhui Agricultural University, Hefei 230036, China; Anhui Engineering Research Center of Functional Food for Plant Active Peptides, Hefei 230036, Anhui, China; College of Tea and Food Science & Technology, Anhui Agricultural University, Hefei 230036, Anhui, China; Key Laboratory of Jianghuai Agricultural Product Fine Processing and Resource Utilization, Ministry of Agriculture and Rural Affairs, Anhui Agricultural University, Hefei 230036, China
Mengdan Xu: Anhui Engineering Laboratory for Agro-products Processing, Anhui Agricultural University, Hefei 230036, China; Anhui Engineering Research Center of Functional Food for Plant Active Peptides, Hefei 230036, Anhui, China; College of Tea and Food Science & Technology, Anhui Agricultural University, Hefei 230036, Anhui, China; Key Laboratory of Jianghuai Agricultural Product Fine Processing and Resource Utilization, Ministry of Agriculture and Rural Affairs, Anhui Agricultural University, Hefei 230036, China
Dawei Cheng: Anhui Engineering Laboratory for Agro-products Processing, Anhui Agricultural University, Hefei 230036, China; Anhui Engineering Research Center of Functional Food for Plant Active Peptides, Hefei 230036, Anhui, China; College of Tea and Food Science & Technology, Anhui Agricultural University, Hefei 230036, Anhui, China; Key Laboratory of Jianghuai Agricultural Product Fine Processing and Resource Utilization, Ministry of Agriculture and Rural Affairs, Anhui Agricultural University, Hefei 230036, China
Xueling Li: Anhui Engineering Laboratory for Agro-products Processing, Anhui Agricultural University, Hefei 230036, China; Anhui Engineering Research Center of Functional Food for Plant Active Peptides, Hefei 230036, Anhui, China; College of Tea and Food Science & Technology, Anhui Agricultural University, Hefei 230036, Anhui, China; Key Laboratory of Jianghuai Agricultural Product Fine Processing and Resource Utilization, Ministry of Agriculture and Rural Affairs, Anhui Agricultural University, Hefei 230036, China
Meiqing Li: Corresponding author at: Anhui Engineering Laboratory for Agro-products Processing, Anhui Agricultural University, Hefei 230036, China.; Anhui Engineering Laboratory for Agro-products Processing, Anhui Agricultural University, Hefei 230036, China; Anhui Engineering Research Center of Functional Food for Plant Active Peptides, Hefei 230036, Anhui, China; College of Tea and Food Science & Technology, Anhui Agricultural University, Hefei 230036, Anhui, China; Key Laboratory of Jianghuai Agricultural Product Fine Processing and Resource Utilization, Ministry of Agriculture and Rural Affairs, Anhui Agricultural University, Hefei 230036, China

Journal volume & issue: Vol. 106
p. 105604

Abstract

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The main purpose of this study was to isolate and identify Angiotensin Converting Enzyme (ACE) inhibitory peptides (IPs) from black soybean protein hydrolysate and compare the activity between single and mixed peptides. Black soybean protein was hydrolyzed, desalted, and separated by size-exclusion ultrafiltration and G-15 Sephadex column. A total of 31 peptides were identified by nano-HPLC-ESI-MS/MS. 7 peptides were screened by molecular docking, only three were ACEIPs after synthetized and bioactive verified, namely KDFPPR (IC50 = 7.91 ± 0.27 mg/mL), VVPPGHPF (IC50 = 12.13 ± 0.38 mg/mL) and DTFPYPR (IC50 = 10.69 ± 0.28 mg/mL), 3 peptides all show mixed-type mode of enzyme inhibition. The synergistic effect on the inhibition of ACE activity was shown among the three ACEIPs. The other four peptides play an auxiliary role in the activity of three ACEIPs mixtures. This study showed that black soybean protein is a reliable source of ACEIPs and the synergism of natural mixed peptides may play an important role in the activity of bioactive peptides.

Published in Journal of Functional Foods

ISSN: 1756-4646 (Print); 2214-9414 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.journals.elsevier.com/journal-of-functional-foods

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